The effects of bioregulators of stem and progenitor cells (BSPC) of fetal tissue cytosol on rat liver during 24h hypothermic storage (HS) and following normothermic reperfusion (NR) were investigated. It was shown that BSPC presence in the preservation medium stabilized pro‐oxidant‐antioxidant balance impaired in liver after HS and NR, prevented the uncoupling of mitochondrial oxidative phosphorylation and ATP level decline. These consequences led to significant improvement of hepatic morphological integrity and functional state. Powerful protective effect of BSPC on liver at sub‐zero temperatures can serve as basis for new approaches to extend safe time for organ preservation and foster understanding of pathways of stem and progenitor cell paracrine action. © 2016 BioFactors, 42(3):287–295, 2016
During the erythrocyte transfusion, a significant part of the cells (~ 25%) is utilized by the spleen and liver in a day due to the deformations and damage to cell membranes that resulted from the hypothermic storage or cryopreservation. An increase in free iron in the blood during the decomposition of red blood cells leads to the stimulation of oxidative stress and inflammation, as well as to dysfunction of the main organs. The primary indicator of damage to the membranes of erythrocytes can be detected through the changes in their morphology. The work investigated the morphological characteristics of erythrocytes after cryopreservation in a medium containing PEG-1500 and 1,2-PD. Erythrocytes washed after thawing and suspended in an isotonic NaCl solution are represented by echinocytes, at the same time, when placed into a solution with albumin, the cells demonstrate the restoration of their discoid shape. According to the well-established concept, the regulation of the shape of erythrocytes is determined by a change in the conformation of the anionic carrier, which is formed by the main integral membrane protein, the protein of band 3. The study has shown that the treatment of erythrocytes with DIDS, which leads to the fixation of the conformation of the carrier with the transport site facing the outer side of the membrane, does not change the morphomodulatory effect of albumin. At the same time, with a decrease in the medium pH, when the albumin charge approaches zero, its effect is eliminated. The results obtained suggest that, upon freezing-thawing of erythrocytes, the deterioration of hydrophobic interactions of transmembrane segments of the protein of band 3 is accompanied by a change in the balance of electrostatic interactions. Probably, the action of albumin is carried out due to the restoration and stabilization of the indicated balance of membranes, charactestic of the typical eruthrocyte discoid shape. Restoration of the morphology of cryopreserved erythrocytes before the transfusion can help reduce cell deformation, improve systemic hemodynamics and prevent the development of negative clinical consequences.
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