Agaricinic acid was extracted from the carpophore of Fomitopsis officinalis (Vill. Bond. et Sing). Its structure was established by 1 H and 13 C NMR spectroscopy and comparison to a standard sample (Sigma-Aldrich). The extraction was carried out using ethanol. The obtained extract was evaporated, cooled (-5°C), and purified by ether. The structure of unbranched alkyl radicals was determined using their proton spectrum. The 13 C NMR spectrum and two-dimensional { 13 C, 1 H} correlation diagram showed the presence of a hydroxyl (second quaternary C atom) and three types of carboxy groups (first, second, and third C atoms). The spectrum of the extracted sample is identical to that of the standard sample of agaricinic acid.
The conditions of extraction of the sum of free amino acids from pollen (pollen pellet) have been studied. A spectrophotometric technique for the quantitative determination of free amino acids in pollen, which is based on the reaction with ninhydrin, is developed. The relative error of analysis using the proposed method does not exceed ± 3.4% at a confidence probability of 0.95. 82 0091-150X/06/4002-0082
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