The present study was conducted to investigate plasma macrominerals (Ca, P, Na, K, and Mg) in adult male and female quails according to different body weights in four close-bred flocks of Japanese quails (Coturnix coturnix japonica) maintained at the Avian Research and Training Center of the University of Veterinary and Animal Sciences, Lahore. The results showed that mean plasma Na concentration was significantly (P < 0.05) different in female quails with respect to body size categories. The interaction between flocks and body size for plasma Ca levels was significant (P < 0.05) in both sexes of quails, whereas plasma Na was significant (P < 0.05) in female quails. The mean plasma P and K levels in imported and local flocks of Japanese quails were significantly (P < 0.05) different in female quails, whereas plasma Mg was significantly (P < 0.05) different in male quails. However, with respect to body size categories, plasma P, K, and Mg were significantly (P < 0.05) different in female quails only. The interaction between flocks and body size was significant for K and P in female quails and for plasma Mg levels in both sexes of quails.