Oxidized phospholipids play essential roles in execution of mitochondrial stage of apoptosis and clearance of apoptotic cells. The identification and quantification of oxidized phospholipids generated during apoptosis can be successfully achieved by oxidative lipidomics. With this approach, diverse molecular species of phospholipids and their hydroperoxides are identified and characterized by soft-ionization mass-spectrometry techniques such as electrospray ionization (ESI). Quantitative assessment of lipid hydroperoxides is performed by fluorescence HPLC-based protocol. The protocol is based on separation of phospholipids using two-dimensional-high-performance thin-layer chromatography (2-D-HPTLC). Phospholipids are hydrolyzed using phospholipase A(2). The fatty acid hydroperoxides (FA-OOH) released is quantified by a fluorometric assay using Amplex red reagent and microperoxidase-11 (MP-11). Detection limit of this protocol is 1-2 pmol of lipid hydroperoxides. Lipid arrays vs. oxidized lipid arrays can be performed by comparing the abundance of phospholipids with the abundance of oxidized phospholipids. Using oxidative lipidomics approach we show that the pattern of phospholipid oxidation during apoptosis is nonrandom and does not follow their abundance in several types of cells undergoing apoptosis and a variety of disease states. This has important implications for evaluation of apoptosis in vivo. The anionic phospholipids, cardiolipin (CL) and phosphatidylserine (PS), are the preferred peroxidation substrates.
Визначено чинники від яких залеж ить чутливість мікроорганізмів до полігексаметиленгуанідину (ПГМГ). Випробувано солі ПГМГ хлорид, валерат, малеат, сукцинат. О б 'єктами К л ю ч о в і с л о в а: бактерії, грибки, віруси, чутливість, полігексаметиленгуанідин, дезінфектант, фосфоліпіди.
This article analyses the results of studying the influence of polymeric guanidine derivatives, namely polyhexamethyleneguanidine chloride (PНMG), on land and freshwater plants. Gas chromatography was used to determine the soil-plant and water-plant transition coefficients. Methods of mass spectrometry and photocalorimetry were used to identify the PHMG in the samples. We investigated the toxicity of PНMG for freshwater flora in an aquarium on Vallisneria spiralis, Riccia fluitans and Chlorella pyrenoidosa. The results showed that even the lowest bactericidal concentrations of the preparation (10-3 % or 10 mg/L) caused the death of test organisms within one to two days. Onetime application of PНMG to the aquarium in dose of 10-4 % (or 1 mg/L) did not cause any noticeable changes in algae during the 7 days of the experiment. The PНMG transfer coefficient did not exceed 0.1% for the system "water-algae tissue". Moreover, the initial concentration of the drug in the water decreased by almost ten times already during the first two days. PНMG polycation molecules quickly bind to dissolved in water organic and inorganic substances, suspended particles, microorganisms, etc. Flocculation causes a sharp decrease in the number of active "free" polycation molecules in water. The drug settles on the bottom of the aquarium and then is destroyed by bacteria saprophytes. Apparently, PHMG is included in their metabolism and serves as nitrogen source for microorganisms Pseudomonas putida, Flavobacterium columnare, Bacillus sp., Sarcina sp., Nitrosomonas sp. and Nitrobacter sp. At the same time, this study showed that the safe concentration of PHMG for hydrobionts in the water of natural fresh water reservoirs is 0.01 mg/l, or 10-6 %, provided the drug is chronic. Ground plants are more resistant to the action of PHMG. They easiliy tolerate finely dispersed spraying with 0.3% aqueous solution of PGMG chloride in a dose of 0.5-1.0 l/m 2. For Urtica dioica, Artemisia absinthium, Taraxacum officinale and Poa angustifolia the coefficients of transfer of the preparation from the surface of plants to internal tissues did not exceed 0.01%. And the coefficients of transfer of PHMG from soil to plants were in the range of 0.004-0.008%. We conducted environmental monitoring in Rivne region during 2011-2015. It showed that numerous cases of use of PGMG drugs for disinfection of various agricultural objects did not lead to any noticeable negative consequences for natural biocenoses. Consequently, we can assume that the preparations of PНMG do not pose a serious threat to the vegetative component of ecosystems. Migration of the drug is minimal in food chains in soil and water.
Biological pollution of natural phytocenoses by adventive plant species poses is a serious threat to endemic species and species with narrower ecological amplitude in ecosystems around the world. This study presents the results of a study of the composition and distribution of adventive plant species in natural, semi-natural and anthropogenic transformed phytocenoses of Volyn’ Polissya, Ukraine. To clarify the effect of drainage melioration on non-native flora species distribution, a botanical study was carried out during the 2003–2019 vegetation seasons on the territory of four drainage systems. The adventive flora of this unique region of Europe was studied in detail for the first time. In total, 279 non-native plant species were found. They belong to 110 genera and 32 families. The results of studying the systematic, bioecological, range-distributional and phytogenetic structure of adventive species found within agricultural lands and adjacent territories are presented. It was found that 161 species are associated with agricultural production. Of these, 90 species were found directly within the agricultural land, another 71 species were found growing in the adjacent territories. It was found that a significant increase in the number of adventive species on the territory of Volyn’ Polissya is associated with drainage melioration carried out in the 1960–1990s, significant changes in the structure and forms of agro-industrial production, and climatic changes in recent decades. The expansion of agricultural land in this area over the past 50–60 years has led to an increase in the number of adventive species by more than 60%. On the territory of drainage systems used for agricultural activities, more than 40% of the total species composition of the adventive flora of Volyn’ Polissya is represented. The majority of these species originate from arid and sub arid regions of the planet. The transformer plants, Solidago canadensis L. and Phalacroloma annuum (L.) Dumort pose a potential threat to phytocenoses of the described region, as well as the whole of Europe. Of particular interest are the species that in the future may pose a serious threat to natural phyto-diversity and have negative practical consequences for the structure of agricultural landscapes. These are Ambrosia artemisiifolia L. and Heracleum sosnowskyi Manden. In general, modern agro ecosystems are characterized by instability and low ability to resist non-native species. The strongly weedy character of cultivated fields and the presence of abandoned uncultivated lands have caused the rapid spread of adventive vegetation. In order to further optimize the structure of agrolandscapes, it is advisable to monitor and regulate not only expansionary invasive species, which is especially important for preventing biological pollution, but also species whose status has not yet been determined.
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