We report here on the genome sequence of Pasteurella multocida Razi 0002 of avian origin, isolated in Iran. The genome has a size of 2,289,036 bp, a GC content of 40.3%, and is predicted to contain 2,079 coding sequences.

We report on our search for microlensing towards the Large Magellanic Cloud (LMC). Analysis of 5.7 years of photometry on 11.9 million stars in the LMC reveals 13 -17 microlensing events. A detailed treatment of our detection efficiency shows that this is significantly more than the ∼ 2 to 4 events expected from lensing by known stellar populations. The timescales ( t ) of the events range from 34 to 230 days. We estimate the microlensing optical depth towards the LMC from events with 2 < t < 400 days to be τ 400 2 = 1.2 +0.4 −0.3 × 10 −7 , with an additional 20% to 30% of systematic error. The spatial distribution of events is mildly inconsistent with LMC/LMC disk self-lensing, but is consistent with an extended lens distribution such as a Milky Way or LMC halo. Interpreted in the context of a Galactic dark matter halo, consisting partially of compact objects, a maximum likelihood analysis gives a MACHO halo fraction of 20% for a typical halo model with a 95% confidence interval of 8% to 50%. A 100% MACHO halo is ruled out at the 95% C.L. for all except our most extreme halo model. Interpreted as a Galactic halo population, the most likely MACHO mass is between 0.15 M ⊙ and 0.9 M ⊙ , depending on the halo model, and the total mass in MACHOs out to 50 kpc is found to be 9 +4 −3 × 10 10 M ⊙ , independent of the halo model. These results are marginally consistent with our previous results, but are lower by about a factor of two. This is mostly due to Poisson noise because with 3.4 times more exposure and increased sensitivity to long timescale events, we did not find the expected factor of ∼ 4 more events. Besides a larger data set, this work also includes an improved efficiency determination, improved likelihood analysis, and more thorough testing of systematic errors, especially with respect to the treatment of potential backgrounds to microlensing. We note that an important source of background are supernovae in galaxies behind the LMC.

We present a new technique for monitoring microlensing activity even in highly crowded fields, and use this technique to place limits on low-mass MACHOs in the haloes of M31 and the Galaxy. Unlike present Galactic microlensing surveys, we employ a technique in which a large fraction of the stellar sample is compressed into a single CCD field, rather than spread out in a way requiring many different telescope pointings. We implement the suggestion by Crotts (1992) that crowded fields can be monitored by searching for changes in flux of variable objects by subtracting images of the same field, taken in time sequence, positionally registered, photometrically normalized, then subtracted from one another (or a sequence average). The present work tackles the most difficult part of this task, the adjustment of the point spread function among images in the sequence so that seeing variations play an insignificant role in determining the residual after subtraction. The interesting signal following this process consists of positive and negative point sources due to variable sources. The measurement of changes in flux determined in this way we dub "difference image photometry" (also called "pixel lensing" [Gould 1996]).The matching of the image point spread function (PSF) is accomplished by a division of PSFs in Fourier space to produce a convolution kernel, in a manner explored for other reasons by Phillips & Davis (1995). In practice, we find the application of this method is difficult in a typical telescope and wide field imaging camera due to a subtle interplay between the spatial variation of the PSF associated with the optical design and the inevitable time variability of the telescope focus. Such effects lead to complexities in matching the PSF

We report on a search for long duration microlensing events towards the Large Magellanic Cloud. We find none, and therefore put limits on the contribution of high mass objects to the Galactic dark matter. At 95% confidence level we exclude objects in the mass range 0.3 M ⊙ to 30.0 M ⊙ from contributing more than 4 × 10 11 M ⊙ to the Galactic halo. Combined with earlier results, this means that objects with masses under 30 M ⊙ cannot make up the entire dark matter halo if the halo is of typical size. For a typical dark halo, objects with masses under 10 M ⊙ contribute less than 40% of the dark matter.

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The nature of dark matter remains mysterious, with luminous material accounting for at most approximately 25 per cent of the baryons in the Universe. We accordingly undertook a survey looking for the microlensing of stars in the Large Magellanic Cloud (LMC) to determine the fraction of Galactic dark matter contained in massive compact halo objects (MACHOs). The presence of the dark matter would be revealed by gravitational lensing of the light from an LMC star as the foreground dark matter moves across the line of sight. The duration of the lensing event is the key observable parameter, but gives non-unique solutions when attempting to estimate the mass, distance and transverse velocity of the lens. The survey results to date indicate that between 8 and 50 per cent of the baryonic mass of the Galactic halo is in the form of MACHOs (ref. 3), but removing the degeneracy by identifying a lensing object would tighten the constraints on the mass in MACHOs. Here we report a direct image of a microlens, revealing it to be a nearby low-mass star in the disk of the Milky Way. This is consistent with the expected frequency of nearby stars acting as lenses, and demonstrates a direct determination of a lens mass from a microlensing event. Complete solutions such as this for halo microlensing events will probe directly the nature of the MACHOs.

We report photometry and spectroscopy of the evolution of Nova Sagittarii 1994 #1 (V4332 Sagittarii) during outburst. We compare the photometric and spectral evolution of this outburst to known classes of outbursts -including classical novae and outbursts occurring on symbiotic stars -and find this object does not conform to any known class of outburst. The closest match to the behavior of this unusual object is M31 RV, an extremely luminous and red variable object discovered in the bulge of M31 in 1988. However, the temporal behavior and maximum luminosity of the two events differ by several orders of magnitude, requiring substantial intrinsic variation if these two events are members the same type of outburst.Our model of the spectroscopic evolution of this outburst shows that the effective temperature cooled from 4400 K to 2300 K over the three month span of our observations. In combination with line diagnostics in our later spectra, including [OI] λ5577 and the dramatic increase in the Hα to Hβ ratio, we infer the existence of a 1 Postal Address: Steward Observatory, University of Arizona, Tucson, AZ 85721 cool, dense (N e ∼ 10 8−9 cm −3 ) envelope that is optically thick in the Hydrogen Balmer recombination lines (case C). We suggest that a nuclear event in a single star, in which a slow shock drove the photosphere outwards, can power the observed luminosity evolution and the emission spectrum.

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