GS, NI and RHI histopathological scoring systems are comparable in what concerns patients' stratification into histological remission/activity. Additionally, FC levels are increased when neutrophils are present in the epithelium and the intestinal mucosa has erosions or ulcers. The presence of neutrophils in the epithelium is, indeed, the main marker of histological activity.

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ResumoO pequi é um fruto nativo do Cerrado que apresenta uma grande diversidade em suas características físicas e químicas. Estudos com frutos oriundos de diferentes ambientes de Cerrado são escassos na literatura. O objetivo deste estudo foi analisar as características físicas e nutricionais de frutos de pequizeiro oriundos dos estados de Tocantins (TO), Goiás (GO) e Minas Gerais (MG). Realizou-se a caracterização física de 30 frutos de pequi selecionados aleatoriamente, por estado, e determinou-se a composição química de sua polpa. Os frutos de pequi oriundos de MG apresentaram valores elevados para características físicas de importância comercial, como massa dos frutos, massa da polpa e rendimento de semente (amêndoa). Entretanto, os frutos oriundos de GO apresentaram alto rendimento de polpa. Foi observado conteúdo reduzido de lipídios nos frutos de TO (8 g.100 g -1 ), ao contrário dos frutos provenientes de GO e MG (24 e 26 g.100 g -1 , respectivamente). Em relação ao conteúdo de minerais, a polpa de pequi dos três estados constitui fonte de ferro, zinco, fósforo e magnésio, e a polpa de pequi oriundo de TO apresentou teor considerável de cálcio (107 mg.100 g -1 ). Conclui-se que a região de origem infl uencia nas características físicas e químicas dos frutos, sobretudo no rendimento de polpa, nos teores de umidade e lipídios, na densidade energética e no conteúdo de cálcio da polpa de pequi. Esses resultados indicam a necessidade de caracterização da região de origem e das espécies de pequizeiro para a comercialização dos frutos e utilização da polpa como matéria-prima nutritiva em diferentes sistemas alimentares. Palavras-chave: Caryocar brasiliense; Cerrado; Frutíferas nativas; Composição química. SummaryPequi is a native fruit from the Brazilian Cerrado with a great diversity in its physical and chemical characteristics. Studies with fruits from different environments of the Cerrado are scarce. The objective of this study was to analyze, physically and nutritionally, pequi fruits from Tocantins (TO), Goiás (GO) and Minas Gerais (MG) States. The physical analysis of 30 pequi fruits, randomly selected by region, was carried out, and the chemical composition of their pulps was determined. The pequi fruits from MG showed high values of commercially important physical characteristics, as fruit mass, pulp mass and seed (almond) yield. However, fruits from GO showed high pulp yield. Low lipid contents (8 g.100g -1 ) were observed in the fruits from TO, on the contrary of the fruits from GO and MG (24 and 26 g.100g -1 , respectively). Regarding the mineral content, the pequi pulp from the three regions is a source of iron, zinc, phosphorus and magnesium, and the pequi pulp from TO showed considerable contents of calcium (107 mg.100g -1 ). The native region of the fruits infl uences their physical and chemical characteristics, mainly the pulp yield, moisture and lipid contents, energy density and calcium amount of the pequi pulp. These results indicate the necessity of characterization of the native region and...

Leprosy affects skin and peripheral nerves, and acute inflammatory type 1 reactions (reversal reaction) can cause neurologic impairment and disabilities. Single skin lesion paucibacillary leprosy volunteers (N = 135) recruited in three Brazilian endemic regions, treated with single-dose rifampin, ofloxacin, and minocycline (ROM), were monitored for 3 years. Poor outcome was defined as type 1 reactions with or without neuritis. IgM anti-phenolic glycolipid I, histopathology, Mitsuda test, and Mycobacterium leprae DNA polymerase chain reaction (ML-PCR) were performed at baseline. chi(2) test, Kaplan-Meir curves, and Cox proportional hazards were applied. The majority of volunteers were adults with a mean age of 30.5 +/- 15.4 years; 44.4% were ML-PCR positive. During follow-up, 14.8% of the patients had a poor clinical outcome, classified as a type 1 reaction. Older age (> or = 40 years), ML-PCR positivity, and lesion size > 5 cm were associated with increased risk. In multivariate analysis, age (> or = 40 years) and ML-PCR positivity remained baseline predictors of type 1 reaction among monolesion leprosy patients.

Background:There is scant information on the accuracy of different assays used to measure anti-infliximab antibodies (ADAs), especially in the presence of detectable infliximab (IFX). We thus aimed to evaluate and compare three different assays for the detection of IFX and ADAs and to clarify the impact of the presence of circulating IFX on the accuracy of the ADA assays.Methods:Blood samples from 79 ulcerative colitis (UC) patients treated with infliximab were assessed for IFX levels and ADAs using three different assays: an in-house assay and two commercial kits, Immundiagnostik and Theradiag. Sera samples with ADAs and undetectable levels of IFX were spiked with exogenous IFX and analyzed for ADAs.Results:The three assays showed 81–96% agreement for the measured IFX level. However, the in-house assay and Immundiagnostik assays detected ADAs in 34 out of 79 samples, whereas Theradiag only detected ADAs in 24 samples. Samples negative for ADAs with Theradiag, but ADA-positive in both the in-house and Immundiagnostik assays, were positive for IFX or IgG4 ADAs. In spiking experiments, a low concentration of exogenous IFX (5 µg/ml) hampered ADA detection with Theradiag in sera samples with ADA levels of between 3 and 10 µg/ml. In the Immundiagnostik assay detection interference was only observed at concentrations of exogenous IFX higher than 30 µg/ml. However, in samples with high levels of ADAs (>25 µg/ml) interference was only observed at IFX concentrations higher than 100 µg/ml in all three assays. Binary (IFX/ADA) stratification of the results showed that IFX+/ADA- and IFX-/ADAs+ were less influenced by the assay results than the double-positive (IFX+/ADAs+) and double-negative (IFX-/ADAs-) combination.Conclusions:All three methodologies are equally suitable for measuring IFX levels. However, erroneous therapeutic decisions may occur when patients show double-negative (IFX-/ADAs-) or double-positive (IFX+/ADAs+) status, since agreement between assays is significantly lower in these circumstances.

Background and Aims The histological status of ulcerative colitis [UC] patients in clinical and endoscopic remission has gained space as an important prognostic marker and a key component of disease monitoring. Our main aims were to compare two histological indexes—the continuous Geboes score [GS] and the Robarts Histopathology index [RHI]—regarding their definitions of histological remission and response, and the ability of faecal calprotectin [FC] levels to discriminate between these statuses. Methods This was an analysis of three prospective cohorts including 422 patients previously enrolled in other studies. Results The two continuous scores [GS and RHI] were shown to be significantly correlated [correlation coefficient of 0.806, p < 0.001] and particularly close regarding their definition of histological response: 95% and 88% of all patients classified as having/not having [respectively] histological response according to RHI also did so according to GS. Moreover, median FC levels in patients with histological response were lower than those in patients without histological response [GS: 73.00 vs 525.00, p < 0.001; RHI: 73.50 vs 510.00, p < 0.001]; a similar trend was observed when FC levels of patients in histological remission were compared to those of patients with histological activity [GS: 76.00 vs 228.00, p < 0.001; RHI: 73.50 vs 467.00, p < 0.001]. FC levels allowed us to exclude the absence of histological remission [according to RHI] and absence of histological response [according to RHI and GS], with negative predictive values varying from 82% to 96%. However, optimization of the FC cut-off to exclude the absence of histological remission, as for the continuous GS, falls within values that resemble those of the healthy population. Conclusion The continuous GS and RHI histological scores are strongly correlated in their definitions of histological response. An absence of histological remission could only be excluded at physiological levels of FC.

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Reference values for home BP by height percentiles for age and sex in a non-European population of adolescents are provided.