The potential of biosurfactant PS to permeabilize bacterial cells of Pseudomonas aeruginosa, Escherichia coli, and Bacillus subtilis on growing (in vivo) and resting (in vitro) cells was studied. Biosurfactant was shown to have a neutral or detrimental effect on the growth of Gram-positive strains, and this was dependent on the surfactant concentration. The growth of Gram-negative strains was not influenced by the presence of biosurfactant in the media. Cell permeabilization with biosurfactant PS was shown to be more effective with B. subtilis resting cells than with Pseudomonas aeruginosa. Scanning-electron microscopy observations showed that the biosurfactant PS did not exert a disruptive action on resting cells such that it was detrimental to the effect on growing cells of B. subtilis. Low critical micelle concentrations, tender action on nongrowing cells, and neutral effects on the growth of microbial strains at low surfactant concentrations make biosurfactant PS a potential candidate for application in different industrial fields, in environmental bioremediation, and in biomedicine.
The rhamnolipid biosurfactant PS-17 and its complex with the polysaccharide alginate, both produced by the Pseudomonas sp. S-17 strain, were studied for their antiviral activity against herpes simplex virus (HSV) types 1 and 2. They significantly inhibited the herpesvirus cytopathic effect (CPE) in the Madin-Darby bovine kidney (MDBK) cell line. The investigations were carried out according to the CPE inhibition assay protocol. The suppressive effect of the compounds on HSV replication was dose-dependent and occurred at concentrations lower than the critical micelle concentration of the surfactant. The 50% inhibitory concentration (IC 50 ) of rhamnolipid PS-17 was 14.5 μg/ml against HSV-1 and 13 μg/ml against HSV-2. The IC 50 values of the complex were 435 μg/ml for HSV-1 and 482 μg/ml for HSV-2. The inhibitory effects of the substances were confirmed by measuring the infectious virus yields with the multicycle virus growth experimental design as well: Δlog CCID 50 of 1.84Ð2.0 against the two types of herpes simplex viruses by rhamnolipid PS-17 (20 μg/ml), and a strong reduction of the HSV-2 virus yield under the effect of the alginate complex at a concentration of 450 μg/ml. The results indicate that rhamnolipid PS-17 and its alginate complex may be considered as promising substances for the development of anti-herpetic compounds.
Aim: The aim of this work was to study the effect of the novel surfactant PS from Pseudomonas sp. S-17 on Saccharomyces cerevisiae 83±20 yeast cells and to compare it with the effect of the well known surfactant Triton X-100. Methods and Results: The effect of surfactants was investigated on the cells during growth, and on the separated cells. The cell-permeabilizing effect of surfactants was studied by following the release of protein and some enzyme activities. The biosurfactant did not affect the culture growth kinetics, and altered the polypeptide pro®les of cells and membrane proteins in the same way as Triton X-100. Conclusion:Results of this study demonstrate that biosurfactant PS and Triton X-100 have a similar type of action, mainly surface located, and that they do not affect the intracellular structures of yeast cells.
The development of new efficient cleaning technologies for the bioremediation of the environment and its cleaning from oil products has become a subject of great interest. Surfactants influence the bioremediation of oil and other organic contaminants through increasing their availability. Since surfactant products of a microbial nature are highly efficient in this respect, they have important advantages over synthetic materials. Being biodegradable, they are ecologically safe. A new washing bioremedy based on surfactant products synthesized microbially from the bacterial strain Pseudomonas sp. PS-17 has been obtained. It contains an unique biocomplex of a glycolipid biosurfactant and an alginate biopolymer and possesses a high surface and emulsifying activity. The washing and antisorptional capacity of this bioremedy has been tested on objects of various natures, viz. bird feathers, animal skins and furs. We have obtained positive results in the remediation of sea sands, birds and animals suffering from ecological catastrophy. It was shown that the bioremedy has mild properties and does not change the structure of the feathers, skins and furs studied. The bioremedy remains active over the temperature range 15–90°C.
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