A modernized device intended for diagnosing the temperature dependent activity of cells is described. The activity of cells was determined by finding the correlation coefficient of optical signals in speckle patterns recorded at different moments of time. It is shown that, as the temperature increases from 26 to 36°C, variations in the optical paths in cells increase from 16 to 26 nm. This is probably associated with the physiological activity of cells-trapping of large molecules or their conglomerates. In contrast to the pre vious variant of the device design, instead of a lipid membrane, a cell monolayer was used, which was culti vated on a plane-parallel glass plate. In order to reduce variations in the optical path lengths in a nutrient solution, a 1.7 mm thick plane-parallel glass plate was additionally inserted into the cuvette. The modern ization of the device also includes heating of the cuvette with hot air flows.
Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities.The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. The target of the research was cells from L-41 culture placed onto a special optical tray with a nutrient solution immediately after defrosting. Time-average value T of digital radiation intensity in the TV camera pixels I (1) and the correlation coefficient of two digital images η (2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments.It is shown that in the area containing hundreds of cells dependence ( ) t η levels off, which indicate stationarity of random value Ĩ .Features of ( ) t η dependences obtained by averaging over large and small cell numbers image are discussed.Using the data obtained, we formulated recommendations on selecting area sizes for averaging physical values to study various processes occurring in cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.