'Leghorn', 'Cornish' and 'White Rock' hens were subjected to starvation. Free amino acids were determined in blood samples taken after 48, 72 and 96 h of starvation. A progressive decrease in concentration of the majority of amino acids was found. Changes in amino acid concentrations during starvation were dependent on the breed of hen.
Free amino acids were estimated in the plasma of Leghorn, Cornish and White Rock hens, bred under identical conditions. It was found that the plasma of Leghorn hens had a lower content of amino acids. The differences were especially pronounced for proline, glutamic acid and glycine. It was established that a lower percentage of valine, leucine and isoleucine was typical of Leghorn hens in comparison with Cornish hens. The obtained results indicate that the level of free amino acids in blood plasma is genetically controlled.
The content of free amino acids was determined in erythrocytes of adult 'Leghorn' (Lg, 'White Rock' (WR) and 'Cornish' (Cr) hens, bred under identical conditions. The concentration of total amino acids was twice as high in the erythrocytes as in plasma, amounting to 396 μm/100 ml, 424 μm/100 ml and 475μm/100 ml in 'White Rock', 'Cornish' and 'Leghorn' hens, respectively.Significant differences were found in the ratio of basic amino acids to acidic amino acids. These values were 0.76, 1.75 and 3.19 in 'White Rick', 'Leghorn' and 'Cornish' hens, respectively; in the plasma of all 3 breeds the ratio was 1. Statistically significant interbreed differences were expressed more distinctly in erythrocyte than in plasma amino acid concentrations. For absolute concentrations the differences were significant in the case of 9 amino acids.
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