To assess the antioxidant and anti-inflammatory activities of the powder sample derived from the ethyl acetate fraction of the floral Argemone mexicana L. For antioxidant efficiency, the floral extract was evaluated using 1, 1–diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and the FRC (Ferric Reduction Capacity) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) and the production method of nitric oxide (NO). The powder sample extracted from ethyl acetate fraction of the floral of Argemone Mexicana L showed good antioxidant activity with the comparative standard sample in scavenging DPPH radicals and in FRC assay. In the cell viability (PBMC influenced by LPS) method and the Nitric oxide (NO) assay, this sample showed even able anti-inflammative activities. Such results indicate a significant antioxidant and anti-inflammatory activity in the powder sample obtained from ethyl acetate fraction in the flower of Argemone mexicana L.
Aims: To investigate the antioxidant and anti-inflammatory activities of ethyl acetate extract of Moringa oleifera flowers. Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between April 2017 and January 2018. Methodology: Extraction and fractionation were carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate. The anti-inflammatory effect of the extract was investigated by HRBC membrane stabilization and Albumin denaturation methods. Anti-oxidant effect of the extract was determined by DPPH assay and ABTS method. Results: The dry sample extracted from the ethyl acetate fraction of Moringa oleifera flowers possess highly anti-oxidant activity showed by the DPPH assay and ABTS method and also having anti-inflammatory activity is determined by human red blood cell (HRBC) membrane stabilization and Albumin denaturation methods. However, these effects need to be confirmed using in vivo models and clinical trials before its utilization as a therapeutic agent. Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Moringa oleifera flowers possesses effective anti-oxidant and anti-inflammatory activities.
Objective : To evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Hibiscus sabdariffa L. Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by FRC (Ferric Reducing Capacity) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMC) were stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method. Results : The solid powder obtained from the ethyl acetate fraction from the flower Hibiscus sabdariffa L showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS induced PBMCs) assay and nitric oxide (NO) assay. Conclusion : These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower Hibiscus sabdariffa L have significant antioxidant and anti-inflammatory activities.
Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta. Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method. Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay. Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.
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