The sensitivity of Fragaria seedlings to colchicine is dependent on the plant organ that is treated.Complete immersion of seedlings in a 1.5 ~ colchicine solution results in total lethality, whereas the survival rates were more than 75 ~ even at concentrations of 3.0 ~ when only shoot apices were treated. High proportions of polyploids were isolated by treating shoot apices of seedlings with a 2.0 ~ colchicine solution for 24-28 h. The 'dropper' method is preferred to the 'tube' method as it involves a minimum of manipulation and requires simple equipment. A differential response to colchicine was observed within and between different diploid species, diploid and tetraploid hybrids.
Low temperature and humidity were used for the storage of pollen of four species of Fragaria (2n = 14, 42 and 56) and two species of Potentilla (2n = 14). The stainability of Fragaria pollen was assessed over a number of years by use of aceto-carmine stain and its viability by cross-pollinations; stainability of Potentilla pollen was assessed by acetocarmine. Fragaria pollen so stored remained stainable for seven years and showed the ability to set seed for three years. Over the latter period, aceto-carmine staining gave a reasonable prediction of seed set produced by the stored pollen of Fragaria and so is of value in estimating pollen viability in that genus. Potentilla pollen remained stainable for three years.
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