1 2 It has long been suggested that hydrothermal systems might have provided habitats for the origin 3 and evolution of early life on Earth, and possibly other planets such as Mars. In this contribution 4 we show that most impact events that result in the formation of complex impact craters (i.e., >2-5 4 and >5-10 km diameter on Earth and Mars, respectively) are potentially capable of generating 6 a hydrothermal system. Consideration of the impact cratering record on Earth suggests that the 7 presence of an impact crater lake is critical for determining the longevity and size of the 8 hydrothermal system. We show that there are six main locations within and around impact 9 craters on Earth where impact-generated hydrothermal deposits can form: 1) crater-fill impact 10 melt rocks and melt-bearing breccias; 2) interior of central uplifts; 3) outer margin of central 11 uplifts; 4) impact ejecta deposits; 5) crater rim region; and 6) post-impact crater lake sediments. 12We suggest that these six locations are applicable to Mars as well. Evidence for impact-13 generated hydrothermal alteration ranges from discrete vugs and veins to pervasive alteration 14 depending on the setting and nature of the system. A variety of hydrothermal minerals have been 15 documented in terrestrial impact structures and these can be grouped into three broad categories: 16(1) hydrothermally-altered target-rock assemblages; (2) primary hydrothermal minerals 17 precipitated from solutions; and (3) secondary assemblages formed by the alteration of primary 18 hydrothermal minerals. Target lithology and the origin of the hydrothermal fluids strongly 19 influences the hydrothermal mineral assemblages formed in these post-impact hydrothermal 20systems. There is a growing body of evidence for impact-generated hydrothermal activity on 21 Mars; although further detailed studies using high-resolution imagery and multispectral 22 information are required. Such studies have only been done in detail for a handful of Martian 23 4 craters. The best example so far is from Toro Crater (Marzo et al., 2010). We also present new 1 evidence for impact-generated hydrothermal deposits within an unnamed ~32-km diameter crater 2 ~ 350 km away from Toro and within the larger Holden Crater. Synthesizing observations of 3 impact craters on Earth and Mars, we suggest that if there was life on Mars early in its history, 4 then hydrothermal deposits associated with impact craters may provide the best, and most 5 numerous, opportunities for finding preserved evidence for life on Mars. Moreover, 6hydrothermally altered and precipitated rocks can provide nutrients and habitats for life long 7 after hydrothermal activity has ceased. 8 5 1

Life can persist under severe osmotic stress and low water activity in hypersaline environments. On Mars, evidence for the past presence of saline bodies of water is prevalent and resulted in the widespread deposition of sulfate and chloride salts. Here we investigate Spotted Lake (British Columbia, Canada), a hypersaline lake with extreme (>3 M) levels of sulfate salts as an exemplar of the conditions thought to be associated with ancient Mars. We provide the first characterization of microbial structure in Spotted Lake sediments through metagenomic sequencing, and report a bacteria-dominated community with abundant Proteobacteria, Firmicutes, and Bacteroidetes, as well as diverse extremophiles. Microbial abundance and functional comparisons reveal similarities to Ace Lake, a meromictic Antarctic lake with anoxic and sulfidic bottom waters. Our analysis suggests that hypersaline-associated species occupy niches characterized foremost by differential abundance of Archaea, uncharacterized Bacteria, and Cyanobacteria. Potential biosignatures in this environment are discussed, specifically the likelihood of a strong sulfur isotopic fractionation record within the sediments due to the presence of sulfate reducing bacteria. With its high sulfate levels and seasonal freeze-thaw cycles, Spotted Lake is an analog for ancient paleolakes on Mars in which sulfate salt deposits may have offered periodically habitable environments, and could have concentrated and preserved organic materials or their biomarkers over geologic time.

The conditions, timing, and setting for the origin of life on Earth and whether life exists elsewhere in our solar system and beyond represent some of the most fundamental scientific questions of our time. Although the bombardment of planets and satellites by asteroids and comets has long been viewed as a destructive process that would have presented a barrier to the emergence of life and frustrated or extinguished life, we provide a comprehensive synthesis of data and observations on the beneficial role of impacts in a wide range of prebiotic and biological processes. In the context of previously proposed environments for the origin of life on Earth, we discuss how meteorite impacts can generate both subaerial and submarine hydrothermal vents, abundant hydrothermalsedimentary settings, and impact analogues for volcanic pumice rafts and splash pools. Impact events can also deliver and/or generate many of the necessary chemical ingredients for life and catalytic substrates such as clays as well. The role that impact cratering plays in fracturing planetary crusts and its effects on deep subsurface habitats for life are also discussed. In summary, we propose that meteorite impact events are a fundamental geobiological process in planetary evolution that played an important role in the origin of life on Earth. We conclude with the recommendation that impact craters should be considered prime sites in the search for evidence of past life on Mars. Furthermore, unlike other geological processes such as volcanism or plate tectonics, impact cratering is ubiquitous on planetary bodies throughout the Universe and is independent of size, composition, and distance from the host star. Impact events thus provide a mechanism with the potential to generate habitable planets, moons, and asteroids throughout the Solar System and beyond.

Concurrent osmotic and chaotropic stress make MgCl 2 -rich brines extremely inhospitable environments. Understanding the limits of life in these brines is essential to the search for extraterrestrial life on contemporary and relict ocean worlds, like Mars, which could host similar environments. We sequenced environmental 16S rRNA genes and quantified microbial activity across a broad range of salinity and chaotropicity at a Mars-analogue salt harvesting facility in Southern California, where seawater is evaporated in a series of ponds ranging from kosmotropic NaCl brines to highly chaotropic MgCl 2 brines. Within NaCl brines, we observed a proliferation of specialized halophilic Euryarchaeota, which corresponded closely with the dominant taxa found in salterns around the world. These communities were characterized by very slow growth rates and high biomass accumulation. As salinity and chaotropicity increased, we found that the MgCl 2 -rich brines eventually exceeded the limits of microbial activity. We found evidence that exogenous genetic material is preserved in these chaotropic brines, producing an unexpected increase in diversity in the presumably sterile MgCl 2 -saturated brines. Because of their high potential for biomarker preservation, chaotropic brines could therefore serve as repositories of genetic biomarkers from nearby environments (both on Earth and beyond) making them prime targets for future life-detection missions.

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Deep-sea hypersaline anoxic basins (DHABs) are uniquely stratified polyextreme environments generally found in enclosed seas. These environments select for elusive and widely uncharacterized microbes that may be living below the currently recognized window of life on Earth. Still, there is strong evidence of highly specialized active microbial communities in the Kryos, Discovery, and Hephaestus basins located in the Eastern Mediterranean Sea; the only known athalassohaline DHABs. Life is further constrained in these DHABs as near-saturated concentrations of magnesium chloride significantly reduces water activity (a w ) and exerts extreme chaotropic stress, the tendency of a solution to disorder biomolecules. In this review, we provide an overview of microbial adaptations to polyextremes focusing primarily on chaotropicity, summarize current evidence of microbial life within athalassohaline DHABs and describe the difficulties of life detection approaches and sampling within these environments. We also reveal inconsistent measurements of chaotropic activity in the literature highlighting the need for a new methodology. Finally, we generate recommendations for future investigations and discuss the importance of athalassohaline DHAB research to help inform extraterrestrial life detection missions.

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