Streptococcus iniae, a bacterial infection in barramundi La tes calcarifer'~e p a r t m e n t of Microbiology and Immunology, James Cook University, Townsville, Queensland 4811, Australia '~e p a r t m e n t of Primary Industries, Oonoonba, Townsville, Queensland 4811, Australia ABSTRACT: The cause of ongoing mortality in barramundi Lates calcanfer (Bloch) in seawater culture was identified as Streptococcus iniae by biochemical and physiological tests. This is the first published record of this bacterial species in Australia and the first confirmed report of S. iniae causing mortality in barramundi. The bacterium was highly pathogenic for barramundi when challenged by bath exposure. The pathogen was found to have a LDS, of 2.5 X 105 and 3.2 X 104 colony-forming units at 48 h and 10 d respectively. Experimental challenge of barramundi resulted in high levels of mortality (>40%) within a 48 h period. Ten days after the challenge, S. iniae could not be isolated from kidney, spleen, liver or eye of surviving fish. However, the organism was easily isolated from the brain of both moribund and healthy fish, indicating that barramundi can carry the bacterium asymptomatically.

a b s t r a c tMicrocosm studies were performed to evaluate the survival of Escherichia coli, Salmonella paratyphi and Vibrio parahaemolyticus in water and sediment collected from the freshwater region of Vembanad Lake (9 35 • N 76 25• E) along the south west coast of India. All three test microorganisms showed significantly (p < 0.01) higher survival in sediment compared to overlying water. The survival in different sediment types with different particle size and organic carbon content revealed that sediment with small particle size and high organic carbon content could enhance their extended survival (p < 0.05). The results indicate that sediments of the Lake could act as a reservoir of pathogenic bacteria and exhibit a potential health hazard from possible resuspension and subsequent ingestion during recreational activities. Therefore, the assessment of bacterial concentration in freshwater Lake sediments used for contact and non contact recreation has of considerable significance for the proper assessment of microbial pollution of the overlying water, and for the management and protection of related health risk at specific recreational sites. Besides, assessment of the bacterial concentration in sediments can be used as a relatively stable indicator of long term mean bacterial concentration in the water column above.

Remote sensing offers a cost efficient means for identifying and monitoring wetlands over a large area and at different moments in time. In this study, we aim at providing ecologically relevant information on characteristics of temporary and permanent isolated open water wetlands, obtained by standard techniques and relatively cheap imagery. The number, surface area, nearest distance, and dynamics of isolated temporary and permanent wetlands were determined for the Western Cape, South Africa. Open water bodies (wetlands) were mapped from seven Landsat images (acquired during 1987 – 2002) using supervised maximum likelihood classification. The number of wetlands fluctuated over time. Most wetlands were detected in the winter of 2000 and 2002, probably related to road constructions. Imagery acquired in summer contained fewer wetlands than in winter. Most wetlands identified from Landsat images were smaller than one hectare. The average distance to the nearest wetland was larger in summer. In comparison to temporary wetlands, fewer, but larger permanent wetlands were detected. In addition, classification of non-vegetated wetlands on an Envisat ASAR radar image (acquired in June 2005) was evaluated. The number of detected small wetlands was lower for radar imagery than optical imagery (acquired in June 2002), probably because of deterioration of the spatial information content due the extensive pre-processing requirements of the radar image. Both optical and radar classifications allow to assess wetland characteristics that potentially influence plant and animal metacommunity structure. Envisat imagery, however, was less suitable than Landsat imagery for the extraction of detailed ecological information, as only large wetlands can be detected. This study has indicated that ecologically relevant data can be generated for the larger wetlands through relatively cheap imagery and standard techniques, despite the relatively low resolution of Landsat and Envisat imagery. For the characterisation of very small wetlands, high spatial resolution optical or radar images are needed. This study exemplifies the benefits of integrating remote sensing and ecology and hence stimulates interdisciplinary research of isolated wetlands.

Eight hundred and eighty-¢ve strains of bacterial isolates from various samples associated with the natural habitat of Macrobrachium rosenbergii were screened for their probiotic potential. Two putative probionts namely Bacillus NL110 and Vibrio NE17 isolated from the larvae and egg samples, respectively, were selected for experimental studies and were introduced to the juveniles of M. rosenbergii (0.080 AE 0.001g) through di¡erent modes such as through feed, water and both. The probiotic potential of the above bacteria in terms of improvements in water quality, growth, survival, speci¢c growth rate (SGR), feed conversion ratio and immune parameters was evaluated. The treatment groups showed a signi¢cant improvement in SGR and weight gain (Po0.001). Survival among di¡erent treatment groups was better than that in the control group. There were also signi¢cant improvements in the water quality parameters such as the concentration of nitrate and ammonia in the treatment groups (Po0.05). Improvements in immune parameters such as the total haemocyte count (Po0.05), phenoloxidase activity and respiratory burst were also signi¢cant (Po0.001). It is concluded that screening of the natural micro£ora of cultured ¢sh and shell¢sh for putative probionts might yield probiotic strains of bacteria that could be utilized for an environment-friendly and organic mode of aquaculture.

A checklist of earthworm species hitherto known from Kerala, a constituent of the Western Ghats biodiversity hotspot in India, is presented. In total, 88 species and subspecies are recorded, of which 55 are Kerala endemics, 9 near endemics, 14 exotics and 10 native peregrines. These belong to 26 genera and 9 families. The checklist includes the literature citation to the original description, type locality, any significant subsequent generic placements, and the district distributional pattern for each species/ subspecies.