1. Three types of exogenous DNA inserts, i.e. complete linearised pVIVO2-GFP/LacZ vector (9620 bp), the LacZ gene (5317 bp) and the GFP gene (2152 bp) were used to transfect chicken spermatozoa through simple incubation of sperm cells with insert. 2. PCR assay, Dot Blot hybridisation and Southern hybridisation showed the successful internalisation of exogenous DNA by chicken sperm cells. 3. Lipofection and Restriction Enzyme Mediated Integration (REMI) were used to improve the rate of internalisation of exogenous DNA by sperm cells. 4. Results from dot blot as well as Southern hybridisation were semi-quantified and improved exogenous DNA uptake by sperm cells through lipofection and REMI. Stronger signals were observed from hybridisation of LacZ as well as GFP specific probe with the DNA from lipofected exogenous DNA transfected sperm DNA in comparison with those transfected with nude exogenous DNA.
Tomar, A., Shukla, P.K., Gupta, J., Singh, A., Churchil, R.R. and Sharma, D. 2007. Genetic polymorphism among Red Jungle Fowl and domesticated chicken detected by DNA markers. J . Appl. Anim. Res., 31: 205-
208.
Genetic polymorphism was detected between Red Jungle Fowl (RJF), White Leghorn (WL), Rhodes Island Red (RIR), White Plymouth Rock (WR), Red Cornish (RC), Aseel (AS) and Kadaknath (KN) using 3 DNA marker systems i.e. Randomly Amplified Polymorphic D N A Polymorphism (RAPD, 20 primers), Microsatellite Minisatellite Associated Sequence Amplification ( M M A , 3 primers) and Microsatellite markers (8 primers). Out of 119 bands amplified from the 18 polymorphic RAPD primers
Singh, A., Gupta, J., Churchil, R.R., Sharma, D. and Singh, R.V. 2007. Genetic diversity between red jungle fowl and domestic chicken for BL B2 region. J. Appl. Anim. Res., 31: 69-72.
Genetic polymorphism i n B L B 2 gene between Red Jungle Fowl (RJF) and domesticated chicken through sequence homology comparisons was studied. A 277 bp amplicon representing second exon corresponding to extra cellular domain of BL B 2 gene was amplified using chicken specific primers and sequenced (Accession No DQ885564). Sequence homology comparisons of the 277 bp sequence of R J F showed
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