's Ilosp, London. studies wcre undertaken to assess the value of including base in ORS in (i) an animal model of intestinal secretion and (ii) children with acutc gastroenteritis. After exposure to cholera toxin, rat small intestine was perfused in situ with ORS containing base or identical OR5 in which base was replaced by chloride.
In previous studies we have demonstrated that transplantation of fetal rat intestine into the peritoneal cavity of adult rats is possible and that the transplanted gut undergoes differentiation and maturation. Histology of these transplants showed normal appearance. In a further study we wanted to examine the functional integrity of the transplanted intestine after anastomosis to the intestine of the host. Disaccharidase activities were measured in the transplant and compared to the "control" activities found in the intestine of the host rat. Maltase and sucrase activities of the transplant were approximately 60% of control, lactase activity was the same for transplant and control. The capacities of glucose and amino acid uptake ofthe transplanted epithelium were not different from control. Peristalsis ofthe transplant was measured by electromyographic recordings: slow wave frequency and spike activity were at the same levels as in controls. These reresults demonstrate that the transplanted fetal rat intestine has the structural and functional characteristics of the normal rat small bowel and should be considered as an alternative to the transplantation of adult bowel. Department of Pediatrics, University of Naples Atrial natriuretic peptide (ANPI is involved in the renal regulation of water and electrolyte homeostasis. ANP exerts its effects through the stimulation of the guanylate cyclase (GC)-cCMP system. Conflicting data have been reported concerning the effects and the mechanism of action of ANP in the intestine. Aims of the work were: 11 to see whether ANP affects the intestinal vater end electrolyte transport and 2) to investigate its mechanism of action and namely the binding of ANP to intestinal cells or membranes and its effects on the GC-cGMP system. Intravenous administration of ANP ~0.3uglKgl30minI resulted in a significant shlft of water, sodium and chloride absorption toward secretion in the vivo perfused rdt )ejunum. Such effect was prompt, but of short duration (less than 10 mlnutes on a continued ANP infusion). No effect was observed on potassium transport. The effects of the addltion of 3-28 rat RNP in a concentration range of to on GC and on cCHP vere studied using rat ]e]unal homogenatcs and rat jelunal cells. GC and cGMP were measured at various times after the additlon of ANP. 2 mlnutes after the addition on ANP, a modest (1.5 tines) increase of both GC and cGMP was observed which rapidly returned to basal levels. No specific binding was obtained by incubating 125~-AN~ with basolateral membranes, cells or homogenates prepared from rat jejunum. We conclude that ANP may play a role as a short-llved modulator in the intestinal regulation of water and electrolyte homeostasis. The mechanism of such interaction appears to be indirect, since it does not involve the GC-cGMP system nor is mediated by specific binding sites on the entcrocyte. strain AL55-(which had spontaneously lost the ability to elaborate the ST-like toxin). Mixed cultures vere incubated for 2 hours at 37 C and then plated onto X...
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