Parijoto (Medinilla speciosa) is one of plants known to have antibacterial activity that can be used as bioreductor. The purpose of this study was to determine the antibacterial activity of gold nanoparticles using Parijoto as bioreductor (AuNPsP) and to determine the concentration that had the highest antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus. This research was conducted to prepare gold nanoparticles using reductant infusion of parijoto fruits, with a ratio of reducing agent and gold solution 1:4, 1:6, and 1:8 respectively. Characterization of gold nanoparticles was carried out using UV Vis Spectrophotometer, Particle Size Analyzer (PSA), Fourier Transformed Infrared (FTIR) Spectrophotometer, Transmission Electron Microscope (TEM) then tested for antibacterial investigation against Pseudomonas aeruginosa and Staphylococcus aureus. The morphology, size, and structural properties of synthesized gold nanoparticles were determined with TEM and PSA analysis which showed spherical mono-dispersed structure with an average particle size of 200-450 nm. FTIR analysis reveal that are responsible for the capping and stabilization of synthesized gold nanoparticles. The effectiveness of AuNPsP against Pseudomonas aeruginosa and Staphylococcus aureus increases as the concentration of gold nanoparticle increases. Therefore, the antibacterial investigation revealed that the AuNPsP exhibited good antimicrobial activity.
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