We screened serume levels of insulin and cortisol in 20 postmenopausal women. We performed these hormones determinations according to algorithm of their levels circaseptane oscillation. All examined patients did't complain on climacteric disorders and did't have any endocrinological diseases. We found out 5 types of hormone oscillations: when the concentrations of both hormones were at normal levels (5% cases); when insulin levels decreased from time to time (30% cases); when cortisol levels increased from time to time (35% cases); when during insulin levels' decreasing the cortisol levels has increased (25% cases); when both hormones levels increased from time to time.
Introduction. Phosphatidylethanol (PEth) is a phospholipid slowly forming in cell membranes as a result of the phospholipase D enzymatic reaction between phosphatidylcholine and ethanol. As a result of the analysis of published scientific papers, high performance liquid chromatography with mass spectrometric detection is used to determine phosphatidylethanol as a method with high specificity, accuracy, precision and the ability to determine substances at minimum concentrations, which is fundamental for clinical laboratory diagnostics when monitoring alcohol abuse in the population. Aim. The aim of the study was to develop and validate a method for the quantitative determination of a direct marker of alcohol abuse, phosphatidylethanol, in human blood. Materials and methods. Phospholipid was quantified by high performance liquid chromatography with tandem mass spectrometry. Results of the study and their discussion. The developed method was validated for the following parameters: selectivity, lower limit of quantitation, linearity, sample transfer, accuracy, precision, matrix effect, stability. The analytical range was 0.0025 µmol/L – 3.0 µmol/L; LPCO level PEth 16:0/18:1 0.0025 was equal to µmol/l. Conclusions. The developed method for the quantitative determination of phosphatidylethanol is suitable for use in laboratory diagnostics in order to monitor the population in the analysis of alcohol abuse.
Рассмотрена проблема незаконного добавления лекарственного вещества сибутрамина в биологически активные добавки к пище и их распространения на территории РФ. Цель работы — разработка методик предварительного и подтверждающего определения сибутрамина в БАД к пище. Метод ТСХ используется для проведения скрининга с последующим подтверждением инструментальными методами, такими как ВЭЖХ-МС, ГХ-МС, что позволит контролировать ввоз и оборот незаконно добавленного препарата сибутрамина в БАД к пище. Метанол и компоненты БАД к пище не влияют на обнаружение сибутрамина, что доказывает специфичность методики. С помощью предложенных методик сибутрамин выявлен в 4 образцах БАД к пище из 8.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.