Aim:The current study was the first of its kind taken upon indigenous ecotypes of the Karnataka in order to unravel the diversity details at 20 chicken microsatellite regions.Materials and Methods:210 indigenous chicken belonging to six districts of Bangalore and Mysore division formed the target sample for the present study. The genomic deoxyribonucleic acid was isolated by phenol chloroform isoamyl alcohol method. A panel of 20 microsatellite regions, including 14 recommended by FAO and six identified from published scientific literature became the targeted chicken genomic region. 27-33 samples were successfully genotyped in each of the six ecotypes through simplex or multiplex polymerase chain reactions, polyacrylamide gel electrophoresis and silver staining for the selected microsatellite panel.Results:The chickens of Ramanagara and Chamrajnagara were most distant with a Nei’s genetic distance value of 0.22. The chickens of Bangalore rural and Mysore were least distant with a value of 0.056. The Ramanagara and Chamrajnagara pair had Nei’s genetic identity value of 0.802, which is least among all pairs of ecotypes. There were five main nodes from which the six ecotypes evolved on the basis 20 microsatellite markers used in this study. This study indicates that the four ecotypes Ramnagara, Bangalore Rural, Chickaballapura and Mysore are genetically identical due to their common ancestral evolution while, Mandya and Chamrajnagara ecotypes formed a relatively different cluster due to a separate common ancestral chicken population and less number of generations since drifting from bifurcation node.Conclusion:Twenty microsatellite markers based genetic diversity study on six indigenous ecotypes indicated lower genetic distances as well as lower FST values compared to the distinguished breeds reported. There were two main clusters, which differentiated into six ecotypes. They may differentiate into more distinct varieties if bred in isolation for a longer number of generations.

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The present study was carried out in six indigenous ecotypes of two divisions of Karnataka to assess association of twenty microsatellite regions of thirteen chicken autosomes with age, body weight and egg weight at sexual maturity and Forty week egg production. The general molecular technique protocols were adopted wherever required in PCR, electrophoresis, gel staining and reading. The analysis revealed significant difference (p<0.05) among genotypes combined across ecotypes for nineteen microsatellite loci for body weight at sexual maturity. The analysis revealed significant difference (P<0.05) among genotypes combined across ecotypes for eighteen microsatellite loci for EWSM. The posthoc dunnet's test conducted in one of the microsatellite region ADL0020 genotypes after excluding genotypes with only one bird at 0.05 level of significance revealed that a particular genotype A was significantly different from two of the genotypes C and D, indicating the important role of the corresponding alleles of these genotypes in influencing the Body weight at sexual maturity. The validity of using thus identified markers or alleles need further authentication by research in other populations and further proof by expression studies.

The aim of this study was to evaluate the oxidative status in crossbred cows with subclinical mastitis and concurrent metabolic and infectious diseases. The crossbred cows were groped asGp-C (n=6) as controls,Gp-M (n=6) with clinical mastitis ,Gp-TS consists cows affected withstenosis of teat,Gp-RA with ruminal acidosis,Gp-ND with diarrhea along with dehydration and loss of appetite, Gp-RTIwith coughing, fever, decreased appetite, varying degrees of dyspnea, GpRB with a history of failure to conceive after at least two successive inseminations. oxidative stress parameters likecatalase, SOD, GPx, GSH and malondialdehyde were estimated spectrophotometrically. The antioxidant enzymes like catalase, super oxide dismutase (SOD), Glutathione Peroxidase (GPx) levels were significantly declined and the non enzymatic parameters reduced glutathione (GSH) and malondialdehyde (MDA) are significantly elevated in affected animals than in healthy animals. The results of the oxidative stress parameters indicate imbalance of antioxidant profile and oxidative stress in the animals with various disorders and previous illness along with subclinical mastitis.

:The present study was carried out in six indigenous ecotypes of two divisions of Karnataka to assess association of twenty microsatellite genotypes belonging to thirteen chicken autosomes with Body weight at different ages. The general molecular technique protocols suggested by Sambrook et al. (1989) were adopted wherever required in PCR, electrophoresis, gel staining and reading. The analysis revealed significant difference (p<0.05) among genotypes combined across ecotypes for nineteen microsatellite loci for body weight at sexual maturity. The validity of using thus, identified markers or alleles need further authentication by research in other populations and further proof by expression studies. Considerable numbers of significant associations were identified in later ages (particularly from sixth week) except for first week in earlier ages across all the microsatellite regions explored except MCW007. There was no significant difference among genotypes of any microsatellite regions for traits like day old, second, fourth, fifth and sixth week body weights suggesting absence of definite trend in the influence of microsatellite regions on body weights at different ages in the indigenous chicken ecotypes.Key words : Microsatellite, Association, Significance, Genotypes How to cite this paper : Rudresh, B.H., Kotresh, A.M., Ashok, M. and Murthy, H.N.N. (2016). Association between microsatellite genotypes and body weight at different ages in indigenous chicken ecotypes. Vet. Sci. Res. J., 7(1) : 1-8.