The water quality of the “La Brava” creek, in Ocaña Norte de Santander, was analyzed using physicochemical and biological parameters to determine quality indices. Three sampling sections were established throughout the study area, taking samples in duplicate every four months for each point during a year; characterization of each sample was done following the protocols of standard methods. The information obtained was analyzed through water quality and contamination indices, determining that the water quality of the micro-basin is moderate, and its level of contamination is low; remaining in good homogeneous condition along the entire area. The utilization of a systems of indices allows for a good approach to assess the conditions of the basin, establishing that, currently, anthropic activities have minimal effect on the water resource in the study area. It is important to highlight that, although the level of contamination is low, it is necessary to generate strategies that guarantee the continuous quality and water supply in the area.