Effects of milbemycin D against adult Angiostrongylus cantonensis in rats were examined. The first-stage larval counts in rat faeces (larvae per gram of faeces per female worm recovered, LPG/female) were most conspicuously reduced in the group treated with nine consecutive weekly doses of 5.0 mg/kg. The effect was more marked in the group treated with five or ten successive daily doses of 5.0 mg/kg than the group treated with a single dose of 25.0 or 5.0 mg/kg. Host lung-body weight ratio and number of recovered worms were reduced significantly only in the group treated with five or ten successive daily doses of 5.0 mg/kg. These results suggest that the action of milbemycin D on the reproductive system of the worms might be differentiated from its killing action. The in vitro motility of females recovered from rats medicated with nine consecutive weekly doses of 5.0 mg/kg was inhibited, and almost all females and males were semitransparent and colourless. Results obtained from sectioned worms showed little content in their digestive tracts and uteri. In addition, there were few eggs and first-stage larvae in the lung tissues of treated rats. These suggest that milbemycin D affects the reproductive functions of the worms through an indirect mode of action including paralysis and inhibition in food intake and energy and/or synthetic metabolism.
During a helminthological survey of black coot, Fulica atra (Gruiformes: Rallidae), in Sindh Province of Pakistan, eight specimens of undescribed species of trematode belonging to genus Paramonostomum Lühe, 1909 were recovered from large intestine of the single host bird. Paramonostomum bubaki n. sp. differs from its congeners except P. macrovesiculum Dharejo et al., 2006 by having less attenuated anterior end, a pharynx, a bifurcal genital pore, a longer posttesticular space, a Y-shaped excretory vesicle, number of uterine loops (16), and large size of filamentous eggs. P. bubaki n. sp. resembles P. macrovesiculum Dharejo et al., 2006 collected from Fulica atra from Pakistan by having a pharynx but differs in larger body, smaller esophagus, a bifurcal genital pore, shape of cirrus sac, seminal vesicle and gonads, ceca reaching posterior extremity, and a longer posttesticular space.
The present species was named as Diplotriaena passeri. This name is given to new nematode species on the name of its type host. It was compared with previously known species of the genus Diplotriaena on the basis of their diagnostic characteristics.
The current examination was required to study nematodes of Sindh/Jungle Sparrow (Passer pyrrhonotus, Blyth 1845) from Larkana, Sindh, Pakistan. Fifteen Passer pyrrhonotus were examined for nematode parasites but only one bird has been found infected with five nematodes Diplotriaena monticolae Yamaguti, 1935 (02 ♂, 03 ♀) belonging to family Filariidae; Nematodes were collected from the thoracic cavity of the Passer pyrrhonotus. Results of the present study revealed that Passer pyrrhonotus is a new host record for the Diplotriaena monticolae (Yamaguti, 1935). The present specimen Diplotriaena monticolae is accredited for the first time from Pakistan.
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