Moreira MAR, Longato-Filho A, Taromaru E, Queiroz G, Jubé LF, Pinto SA, Schmitt FC. Investigation of human papillomavirus by hybrid capture II in cervical carcinomas including 113 adenocarcinomas and related lesions. Int J Gynecol Cancer 2006;16:586-590.Hybrid capture is an easy and highly sensitive technique for screening population due to its capacity to detect malignant and premalignant lesions of the cervix. To evaluate its sensitivity, we investigated the frequency of high-risk human papillomavirus (HPV) infection and its correlation with glandular malignant lesions, analyzing a total of 113 cases of adenocarcinomas and related lesions. High-risk HPV was investigated using a hybrid capture II (HC2) assay. Samples were collected in two different ways: either brushed directly from surgical specimens before fixation or collected from the patients. We also investigated the frequency of HPV in squamous malignant lesions, 65 squamous cell carcinomas (SCC) and 66 in situ squamous cell carcinomas (ISSCC), to compare the occurrence of HPV for these lesions. The 113 glandular lesions comprised 62 invasive adenocarcinomas (IAC), 8 in situ adenocarcinomas (ISAC), 26 IAC plus SCC, and 17 adenosquamous cells carcinomas (ASCC). The HPV-positive reactions were as follows: 51 (82.2%) in IAC, 8 (100%) in ISAC, 25 (96.1%) in IAC plus SCC, and 14 (82.3%) in ASCC. HC2-positive results in the squamous malignant lesions were as follows: 58 of 63 (89.0%) for SCC and 94 of 103 (91.2%) for ISSCC.High-risk HPV infection was quite similar for glandular and pure squamous invasive malignant lesions, 82.2% and 89.0%, respectively, indicating that high-risk HPV is also highly prevalent in glandular lesions. Although hybrid capture proved to be an excellent adjunctive technique, we do not believe its results merit replacing the Pap smear as a screening tool.KEYWORDS: adenocarcinoma, cervical intraepithelial lesion, HPV, hybrid capture II, squamous cells carcinoma.Cervical cancer is distributed throughout the world, afflicting 500,000 women annually. The most important risk factor for the development of this condition is the human papillomavirus (HPV) infection. It is believed that 80% of primary cancers begin from preexisting squamous dysplasia. With respect to glandular epithelium malignancies, adenocarcinomas account for about 20% of invasive carcinomas, which probably arise from in situ adenocarcinomas (ISAC) (1) .Interestingly, the incidence of adenocarcinoma is increasing mainly in developed countries (1,2) , and it is currently thought to be more significant in large cities due to the increased probability of HPV infections.Glandular malignant lesions, namely, ISAC, are frequently overlooked because clinical, colposcopic, and cytologic parameters are unfamiliar and bypassed by concomitant squamous alterations. Additionally, more than 50% of ISAC are mixed diseases with a squamous lesion counterpart (3) . Indeed, traditional methods have poor sensitivity to detect precursor lesions of glandular carcinomas. HPV status has a significant ...
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