Autogenous mesothelium was used as venous substitute in ten dogs. Patches of mesothelium of three different origins were grafted into the anterior wall of the common iliac veins (CIV): peritoneum taken from and including the posterior rectus sheath (PRS), simple peritoneum (P) and mesentery (M). Animals were killed after 2, 4, 8, and 16 days and after 3 months. The segments of CIV, including the patches, were removed for study. On light microscopy, the PRS grafts showed a normal mesothelium but marked submesothelial fibrosis. The M and P grafts showed normal mesothelium and only mild fibrous thickening. On scanning electron microscopy, there was a perfect continuity of the mesothelial cells and the normal endothelium at the suture line. In the center of the graft, the cells had become elongated along the axis of blood flow. Fibrinolytic activity (FA) was measured by a standardized fibrin plate technique and quantitated in tissue activator units per gram of tissue (TAU/g). The mean FA of iliac vein specimens was 1101.7 +/- 133.3 TAU/g (mean +/- SEM). The mean FA determined before grafting for each kind of mesothelium was the following: PRS = 418.8 +/- 26.9 TAU/g; P = 873.0 +/- 107.1 TAU/g; M = 1142.3 +/- 91.4 TAU/g where only PRS showed values significantly lower than iliac vein mean FA (P less than 0.001). Postoperatively, the mesothelial FA, after an initial reduction, increased on day 4 and reached values significantly higher than the control values (1445.7 +/- 204.1 TAU/g tissue vs 853.1 +/- 62.3 TAU/g tissue; P less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
Thrombo-resistance of mesothelial grafts was evaluated by replacing the inferior vena cava in 16 dogs. In 10 dogs, treated by antiplatelet aggregation agents, uniform graft thrombosis happened within 15 days. In six other dogs, the addition of an arterio-venous fistula produced conditions of flow (1385 ± 178 ml/min; mean ± s.e.m.) and velocity (17.1 ± 4.5 cm/s) closer to human values and markedly improved the patency rates (four patent over six up to 6 months P < 0.02). Light microscopy and scanning electron microscopy studies performed on the patent grafts showed well preserved mesothelial cells. Mesothelial fibrinolytic activity was 1160 ± 257 tissue activator units/g tissue before and 846 ± 142 activator units/g tissue after implantation (P = n.s.). Prostaglandin synthesis by native mesothelium was respectively 252 ± 103 and 7 ± 3 pg/ml/mg wet tissue/min for 6-keto-PGF1α and TXB2. The synthesis was reduced for 6-keto-PGF1α in the patent grafts but unaltered for TXB2, This work puts forward the suggestion that mesothelium is a promising venous substitute in conditions of high flow.
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