Twenty cysts from five patients with adult polycystic kidney disease were evaluated morphologically by electron microscopy and functionally by cyst fluid chemical analysis in order to correlate the structure with the function of the cyst wall. Thirteen proximal cysts, as defined by cyst fluid/serum sodium ratios of 0.8 to 1.2, were lined by epithelial cells with open or short closed apical junctions that appeared permeable to lanthanum. In contrast, seven distal cysts, as defined by cyst fluid/serum sodium ratios of less than 0.4, were lined by epithelial cells with long closed apical junctions that appeared impermeable to lanthanum. Cell organelles showed no distinction between proximal and distal cysts. Cyst basement membranes appeared abnormal, but there was no consistent pattern. The fluid of proximal cysts contained lower creatinine, potassium, and hydrogen ion, and higher chloride concentrations than did the distal cysts. These studies provide morphologic and chemical evidence consistent with te view that cysts originate from nephrons. Moreover, the maintenance of the epithelial lining and transmembrane solute gradients over many years extending up to and beyond the development of renal insufficiency suggests that the cysts function as nephronsthrughout the life of the patient.

A laboratory based epidemiologic study was done to determine the possible relationship of herpesvirus type 2 to carcinoma of the prostate. A total of 305 patients (224 with benign prostatic hypertrophy and 81 with prostatic carcinoma) who underwent transurethral resection of the prostate was studied. Viral cultures were obtained from urethral swabs and urine preoperatively. Prostatic tissue removed at operation was grown as tissue explants, using a monolayer cell culture technique, and examined by specific immunofluorescence and electron microscopy for evidence of herpesvirus type 2. The sera of the patients also were tested for specific antibody to herpesvirus type 2 by the indirect hemagglutination inhibition test. Although herpesvirus was not isolated 5 per cent of the patients had evidence of herpesvirus type 2 antigen in prostatic tissues by specific immunofluorescence. Electron microscopy failed to reveal the presence of viral particles. There was an increased prevalence of herpesvirus type 2 antibody in patients with carcinoma of the prostate compared to the controls with benign prostatic hypertrophy (p less than 0.05). Although marital status was similar the patients with prostatic cancer tended to marry at an earlier age, have more children, more sexual partners and more exposure to prostitutes than their counterparts with benign prostatic hypertrophy. The demonstration of specific herpesvirus type 2 antibody in patients with prostatic cancer supports an etiologic role for herpesvirus type 2 but further studies are needed to describe more definitively the relationship since the indexes of sexual activity are remarkably high in both groups.

An elongate Waulsortian mud‐mound complex developed at Dovedale on a ramp to the southwest of a developing carbonate platform in Derbyshire during Chadian (early Viséan) times. The complex occupied an area of approximately 6 km2 and grew to a maximum relief of 80 m with longitudinal and transverse valleys developed near the southern margin. Five mound associated facies have been identified: mound core, mound flank (fine), mound flank (coarse), intermound (fine) and intermound (coarse). The mound core facies is a massive skeletal wackestone with comminuted sponge debris, foraminifera, ostracodes and crinoid debris set in a matrix of clotted micrite. The mound flank sediments display moderately inclined bedding surfaces. While the mound flank (fine) contains sponge debris, the mound flank (coarse) is dominated by articulated crinoid columnals, and includes algal‐encrusted micritized intraclasts and coarse peloids. The well‐bedded intermound (fine) facies is bituminous and micritic while the intermound (coarse) facies is composed of skeletal‐peloidal‐intraclast grainstones which locally contain calcified algae. Although the fauna is diverse, the density of colonization by metazoans was low and the supply of macrofossil debris modest. The clotted micrite texture is interpreted as the product of micro‐organisms which precipitated and trapped fine‐grained sediment. The mud‐mound complex is dominated by the bathymetric assemblages B and C proposed by Lees, Hallet & Hibo which on their model of the Belgian Waulsortian, indicate depths of between 220 and 280 m. Intercalation of assemblages B/C and C/D on the northern margin of the complex is interpreted as the result of local storm disturbance. A deep water drift is postulated to explain the NW‐SE alignment of the complex which probably fitted the ‘export model’ of Bosence, Rowlands & Quine. Beneath the sediment surface, phreatic flow eroded unlithified sediments and developed interconnected cavities which were filled by cement and sediment relatively eariy. Mound instability triggered the opening of fissures which filled with crinoid debris, peloids, indurated lithoclasts and micrite.

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Scanning electron microscopy of the bronchiolar neuroepithelial bodies (NEB's) in the neonatal mouse lungs was undertaken and correlated with the accompanying transmission electron microscopy. The NEB's appeared as isolated organoids along the entire length of the bronchioles, and often were located at the branching points. The boundary of the NEB's was outlined by the ciliated and Clara cells. Both granulated cells and modified Clara cells participated in the formation of the NEB. The modified Clara cells covered most of the surface of the NEB leaving only small oval areas for the exposed surfaces of the specialized cells which contained numerous cytoplasmic granules. Short and regular microvilli projected from the exposed surfaces of the granulated cells, while only sparse microvilli of irregular length were seen on the surfaces of the modified Clara cells. This scanning electron microscopy of the NEB's further established these organoids as separate morphological entities. In addition, the findings that the NEB's could be easily identified with the scanning electron microscope and subsequently dissected out for further studies would help future investigations of their functions which are not clearly known.