Summary Model experiments were performed to investigate the nitrogen fixation (C2H 2 reduction) and denitrification (N~O formation) capabilities of Azospirillum spp. in association with wheat. Plants and bacteria were grown together for a week and then assayed for activities. This association performed C2H2 reduction or N20 formation, depending on the concentrations of nitrate and oxygen in the vessels. Both activities depended on the Azospirillum strains used. The newly isolated Azospirillum amazonense strains Y1 and Y6 showed significant C2H2 reduction and low N20 formation in association with wheat under the conditions employed and axe possibly useful in practice. A cell-free preparation from Azospirillum brasilense Sp 7 possessed a cytochrome cd type dissimilatory nitrite reductase.
The role and treatment of foreign overriding mandatory provisions in international contract law have been subject to academic discussions for a long time. This has not changed with the introduction of Article 9 of the Rome I Regulation. In the judgment discussed in this case note, the European Court of Justice addressed some of the contentious issues in relation to Article 9(3) of the Rome I Regulation. This note examines and evaluates the solutions found by the ECJ and puts them into context. It also points out some questions the ECJ did not discuss; these questions remain open for now but will need to be addressed in the future.Keywords: Article 9(3) Rome I Regulation, foreign overriding mandatory provisions, conflict-of-law level consideration, substantive law level consideration, principle of sincere cooperation.Resumen: El tratamiento de las leyes de policía de terceros estados en derecho de contratos internacionales ha sido objeto de la polémica desde hace tiempo. Esto no ha cambiado con la entrada en vigor del artículo 9 del Reglamento Roma I. Con la sentencia comentada el Tribunal de Justicia de la Unión Europea ha tratado algunas de la cuestiones debatidas respecto al artículo 9.3 del Reglamento Roma I. Este comentario analiza, evalúa y pone en contexto las soluciones encontradas por el TJUE. También aborda las cuestiones que no han sido comentadas por el TJUE; estas cuestiones permanecen abiertas por el momento pero deberán ser examinadas en el futuro.Palabras clave: leyes de policía de terceros estados, consideración en nivel conflictual, consideración en nivel sustantivo, principio de cooperación leal.
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