Tylophora indica (Burm. F.) Merr is an endangered plant which can be protected from extinction by its large scale production. Nodal segments of healthy plants are used as explants and cultured on MS Basal medium fortified with different growth regulators. Optimum shoot induction conditions from explants were established. In vitro and in vivo phytochemical test were done by using standard methods for chlorophyll, carbohydrates, proteins, lipids and starch. 3mg/l 2, 4 D showed maximum and success full callus production. Shoot initiation started in 7 days and best shoot regeneration reported with 3 mg/ml BAP in Basal medium. Combination of IBA and NAA in concentration 2 and 4 mg/l respectively proved to be best for root initiation. Concentration of chlorophyll, protein, lipid, carbohydrate, and starch in vitro and in vivo culture are investigated.
A total of 50 human milk samples from mothers of different age groups were collected from different regions of Haryana, India. Hexachlorobenzene (HCB), α-HCH, ß-HCH, p,p'-DDE, p,p'-DDT were found in all samples, p,p'-DDD and y-HCH were present in 92 percent each of the total samples analysed. Mean levels of HCB, total HCH and total DDT were 0.109, 0.322 and 1.561 mg L -1 , respectively on whole milk basis. The average daily intake of organochlorine pesticides (OCP) calculated for the suckling infant was significantly higher in this study when compared with the levels recommended by the WHO.
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