Azithromycin and levofloxacin used for the treatment of bacterial infections. Simple, specific, accurate and precise UV spectroscopy method has been developed and validated for simultaneous determination of azithromycin and levofloxacin in bulk drugs and marketed formulation. The developed method involves solving of simultaneous equations using methanol as solvent where an absorbance maximum for azithromycin and levofloxacin was found to be at 291.92nm and 294.08nm, respectively. The drugs obeyed Beer’s law in the concentration range of 50- 250 μg/ mL & 2 – 10 μg/ mL. The method was validated as per ICH guidelines .The method showed good correlation coefficients (r2) 0.999, indicated good linearity of calibration curve for both the drugs. The recovery of azithromycin and levofloxacin was 98.82% and 97 .74% respectively. The robustness and ruggedness of azithromycin (0.368, 0.285), and levofloxacin (0.556, 0.442). The developed method was found to be accurate, reliable, robust showing LOD 0.012μg/mL and 0.016μg/mL LOQ 0.053μg/mL and 0.058μg/mL for azithromycin and levofloxacin.
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