Background: Aloe vera (Family: Liliaceae) has been used for the treatment of diabetes, skin disorders and as an anti-inflammatory agent. There is increased concern about the side effects of conventional medicine in the treatment of Parkinson’s disease (PD). As A.vera has found to have antioxidative property, it may be a safer alternative. Methods: Parkinson’s disease was induced by administering haloperidol (1 mg/kg i.p. daily x 1 week).The mice of either sex were divided into 06 groups (n =12). 1 st day group mice were given distilled water (orally), 2nd group were administered haloperidol (20 mg/kg i.p.).The 3rd, 4th and 5th groups were administered A.vera (100, 200, and 400 mg/kg/day, orally) respectively, along with haloperidol. Group 6- received Levodopa (30mg/kg, i.p,) along with haloperidol. To evaluate anti-Parkinson effect, hanging wire test, tardive dyskinesia test and hole board test were performed on the1st day and 8th day. One way ANOVA was used to detect statistical significance followed by post-hoc Tukey test. Results: A.vera (200 and 400 mg/kg, p.o.) was found to increase the hanging time significantly (p 0.001) in hanging wire test and significantly decreased (p 0.001) the Vacuous Chewing Movements (VCMS) in tardive dyskinesia test as compared to haloperidol group. A.vera (200 and 400 mg/kg, p.o.) was found to significantly increase (p 0.001) the number of dips and no. of line crossings in hole board test when compared to haloperidol group. Conclusion: The results of the present study conclusively showed that A.vera has beneficial effect in haloperidol induced experimental model of Parkinson’s disease.
Background: Elaeocarpus ganitrus (Family: Elaeocarpaceae), has been used for the treatment of depression, convulsions and asthma. The existing literature is lacking in studies showing anti-parkinson effect of E. ganitrus. There is increased concern about the side-effects of conventional medicine in the treatment of Parkinson's disease (PD). Hence E. ganitrus having anti-oxidative property may be a safer alternative. Methods: To evaluate the anti-parkinson effect of E. ganitrus, rota rod and catalepsy bar tests were used. Assessment of oxidative stress was done by measuring the malondialdehyde (MDA) and reduced glutathione (GSH) levels in the striatal region of the brain. One-way ANOVA was used to detect statistical significance, followed by post-hoc Tukey test. Results: E. ganitrus (200 and 400 mg/kg, p.o.) pretreated groups significantly increased the retention time in rota rod test (p<0.001) and significantly decreased the latency period in catalepsy bar test (p<0.001), when compared with haloperidol treated group alone. E. ganitrus (200 and 400 mg/kg, p.o.) pretreated groups showed significant anti-oxidative effect by causing a decrease in brain MDA levels (p<0.001) and a significant increase in GSH levels (p<0.001). Conclusions: Oxidative stress plays a vital role in the pathophysiology of PD. The results of this study conclusively show that E. ganitrus has anti-oxidant activity and neuroprotective activity in haloperidol experimental model of PD.
Purpose
This study involved the computational and pharmacological evaluation of (E)-2-(4-methoxybenzylidene)cyclopentan-1-one (A2K10).
Methods
In silico studies were conducted through virtual screening. Morris water and Y-maze tests were conducted to evaluate Alzheimer’s disease. Acute epilepsy haloperidol,and hyperalgesia were used to calculate the epilepsy model, with Parkinson’s disease and mechanical allodynia at a dose of 1–10 mg/kg in the mouse model.
Results
A2K10 exhibited the highest binding affinity against α
7
nicotinic acetylcholine receptors (−256.02 kcal/mol). A2K10 decreased escape latency in the Morris water test during different trials. In the Y-maze test, A2K10 dose-dependently increased spontaneous alteration behavior, with maximum effect of 75.5%±0.86%. Furthermore, A2K10 delayed onset of pentylenetetrazole-induced myoclonic jerks and tonic–clonic seizures and decreased duration of tonic–clonic convulsions in mice, with maximum effect of 93.8±5.30, 77.8±2.91, and 12.9±1.99 seconds, respectively. In the haloperidol-induced Parkinson’s disease model, A2K10 significantly prolonged hanging time and reduced tardive dyskinesia. Moreover, A2K10 extended latency in hot-plate hyperalgesia and increased the paw-withdrawal threshold in mechanical allodynia. In toxicity studies, no mortality was observed.
Conclusion
Overall, the results indicated that A2K10 has potential as an anti-Alzheimer’s, antiepileptic, antiparkinsonian, and analgesic therapeutic compound.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.