Rice transformation was carried out using Agrobacterium strain C58C1 (pGV2260::pSSJ1A) containing a binary vector, pC0390-ubi-rtp-cry2AX1 with a view to generate marker-free transgenic rice plants, resistant to lepidopteran insects. Eight putative transgenic rice plants, positive to gusA and hpt genes, were generated. Four out of the eight putative transgenic plants were positive for presence of cry2AX1 gene, indicating co-transformation of the selectable marker gene, hpt and the gene of interest, cry2AX1 in these plants. Cry2AX1 protein concentration in PCR positive T 0 Plants ranged from 0.010 to 0.022 µg/g of fresh leaf tissue. The T 2 generation plants were subjected to ELISA, and Cry2AX1 protein concentration ranged from 0.016 to 0.057 µg/g of fresh leaf tissue. Insect bioassay studies on ELISA positive T 0 and T 2 plants against neonates of leaffolder resulted in larval mortality ranging from 15 to 30 %
This study was taken up to assess the chlorophyll spectrum induced by Sodium Azide mutagen in two rice cultivars-BPT 2231 and CO 51. The mutagenic effectiveness and efficiency of Sodium Azide across the two genotypes were also observed. The chlorophyll spectrum observed in M 2 generation of BPT 2231 is consisted of albino, xantha, chlorina, striata and xantha viridis. In CO 51, albino and xantha occupied the entire chlorophyll spectrum. Albino was predominant in both the varieties with 1.2 per cent in BPT 2231 and 1.15 per cent in CO 51 at 1mM concentration. The mutagenic frequency was higher in BPT 2231 (7.8) than CO 51 (4.0). Considering the efficiency of a mutagen based on lethality, seedling injury and pollen sterility, BPT 2231 recorded higher values 2.79, 1.10 and 0.26 respectively. Like mutagenic frequency, the mutation rate of Sodium Azide was higher in BPT 2231(0.90) than CO 51 (0.40).
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