The composition of normal, hypertrophied, or sodium-loaded rabbit portal anterior mesenteric vein and of normal and sodium-loaded guinea pig taenia coli smooth muscle was measured in cryosections with electron probe analysis, and the effects of wash with cold sodium-free (Lithium) solutions were determined. There was no significant difference in the cytoplasmic, nuclear, or mitochondrial concentrations of any of the measured elements (sodium, potassium, chloride, magnesium, calcium, phosphorus, sulfur) between hypertrophied, sham-operated, or control veins. The cytoplasmic potassium:sodium:chloride ratio in rabbit portal anterior mesenteric vein was 1:0.26:0.46, and the average sodium concentration (198 mmol/kg dry cytoplasmic weight) was nearly twice as high as estimated from ion flux measurements. The cytoplasmic sodium concentration of normal guinea pig taenia coli was 61 mmol/kg dry weight. The existence of a rapidly exchanging, relatively low affinity, and temperature-insensitive component of cytoplasmic sodium efflux was indicated by the reduction in cytoplasmic sodium after washout in cold, sodium-free (lithium or Tris-substituted) solutions. This reduction, by 62% in normal, 71% in sodium-loaded portal anterior mesenteric vein, and 36% in sodium-loaded guinea pig taenia coli smooth muscle, suggests that the lithium wash method can underestimate cell sodium. In sodium-loaded guinea pig taenia coli and portal anterior mesenteric vein smooth muscles, the cytoplasmic sodium analyzed in individual cells showed a bimodal distribution; in cryosections, the cells having the highest sodium and lowest potassium and phosphorus content also had a more electronlucent (light cell) appearance.
Electron probe x-ray microanalysis of the composition of rabbit portal anterior mesenteric vein smooth muscle was performed following sodium loading and washout into sodium-free lithium solutions. Sodium and lithium were also measured with atomic absorption spectrophotometry. Cellular uptake of sodium and loss of potassium during sodium loading were much faster at high (37 degrees C) than at low (2 degrees C) temperature, as was the passive ouabain-resistant uptake of potassium during lithium washout. The loss of sodium at 2 degrees C into lithium solution consisted of two components: a rapid efflux that was complete by 30 minutes, and a slow component that required at least 24 hours for completion. The amount of sodium lost through the first component (approximately 200-300 mmol/kg dry weight) was relatively independent of the amount of sodium loading. The loss of cellular sodium at 2 degrees C, after 30 minutes, was accompanied by a gain of cellular lithium. Ouabain-resistant sodium loss and lithium and potassium uptake were markedly accelerated at 37 degrees C; sodium loss was complete (1200 mmol sodium/kg dry weight lost) by 30 minutes of washout. Sodium-loaded cells also lost chloride ion and gained magnesium during sodium efflux at 37 degrees C. Mitochondrial and nuclear sodium and potassium were correlated with the respective cytoplasmic concentrations during both sodium loading and sodium washout, indicating the relatively rapid equilibration of the monovalent ions between the cytoplasm and organelles. Calcium-free solutions markedly inhibited the ouabain-resistant sodium and chloride ion effluxes and potassium influx in muscles incubated, after sodium loading, in lithium solutions at 37 degrees C. These fluxes could be restored to near normal values by 0.2 mM calcium. The calcium sensitivity of the ouabain-resistant sodium, potassium, and chloride ion fluxes observed in this and other studies raises the possibility that some abnormalities of monovalent ion transport observed in cells of hypertensives are secondary to changes in cellular calcium.
Electron probe microanalysis (EPMA) has been used to study the subcellular distribution of Ca, Na, K, Cl, and Mg in smooth muscle. The EPMA results indicate that the sarcoplasmic reticulum (SR) is the major intracellular source and sink of activator Ca: norepinephrine decreases the Ca content of the junctional SR in portal vein smooth muscle. Mitochondria do not play a significant role in regulating cytoplasmic free Ca2+, but mitochondrial Ca content can be altered to a degree compatible with suggestions that fluctuations in matrix Ca contribute to the control of mitochondrial metabolism. The rise in total cytoplasmic Ca during a maintained, maximal contraction is very much greater than the rise in free Ca2+, and is probably in excess of the known binding sites available on calmodulin and myosin. Cell Ca is not increased in normal cells that are Na-loaded. The non-Donnan distribution of Cl is not due to compartmentalization, but reflects high cytoplasmic Cl. Na-loading of smooth muscle in K-free solutions is temperature dependent, and may exhibit cellular heterogeneity undetected by conventional techniques. The total cell Mg is equivalent to approximately 12 mM, and less than 50% of it can be accounted for by binding to ATP and to actin. Mitochondrial monovalent cations in smooth muscle are relatively rapidly exchangeable.
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