The dark mudstone lithofacies of Mamu Formation was deposited during the Campano-Maastrichtian flooding episode. It is laterally heterogeneous, and has been subdivided into marsh, bay and central basin subenvironments in order of proximality. Arising from recommendation from a previous study, we evaluated its hydrocarbon generating potential using multidisciplinary tools involving visual kerogen analysis, as well as bulk and isotope geochemistry. Seventy-seven sample materials were taken from 3-outcrop sites at Uzebba, Okpekpe and Imiegba locations, Benin flank, SW Anambra Basin, Nigeria. The results show that bulk of the samples have good organic richness. Kerogen quality is dominantly of gas prone Type III kerogen. However, visual kerogen analysis indicates the presence of an oil prone Type II/III kerogen in the central basin subenvironments. An immature thermal maturity is inferred based on spore colour index (SCI) of less than 6 on the SCI chart (thermal alteration index of <2.5). In addition, we hypothesize that the dark mudstone lithofacies possesses biogenic gas potential based on its organic richness, kerogen quality and thermal maturity. Shale gas prospectivity is further enhanced by the low dip of the Mamu Formation, shallow burial as well as high silica content. Worth mentioning is the proximal marsh mudstone (Uzebba location) with suitable microfabric, very high silica as well as >10m of combined (continuous) outcropping and subcropping thickness Keywords: Kerogen, palynofacies, stable isotope geochemistry, shale gas,
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