Six assays for protein in urine were compared for linearity, ease of standardization, precision, comparability of assay values, technical ease of assay, and current cost. The assays investigated were three dye-binding techniques, a recent turbidimetric technique, the trichloroacetic acid--biuret reaction, and a tannic acid protein precipitation reaction with ferric chloride. All assays suffered from standardization problems, although the biuret method showed the best analytical recovery of albumin and gamma-globulin. The tannic acid/ferric chloride method is dependent on sample pH. The turbidimetric assay exhibited the greatest imprecision; i.e., CVs were 19.5% at a protein concentration of 0.13 g/L and 6.0% at a protein concentration of 1.3 g/L. On the basis of all the factors assessed, we conclude that the Pesce/Strande Ponceau-S and the Bio-Rad Coomassie Brilliant Blue dye-binding techniques offer certain advantages over the other assays studied.
SUMMARY
Oxymetholone 100 mg. per day was given to 60 patients in chronic renal failure, 55 of whom were receiving regular hæmodialysis. Thirty‐nine of the dialysed patients completed a cross‐over double blind trial of oxymetholone, 30 of them taking the active drug for 3 months; there was no significant change in hæmoglobin or hæmatocrit. No significant change occurred in liver function tests and the only side effect that appeared more than occasionally was a characteristic fattening of facial tissues which reverted to normal after withdrawal of the drug.
One patient with polycystic disease treated by Giovannetti diet sustained a rise in hæmoglobin from 8·4 to 10·8 g./100 ml. while taking oxymetholone for 10 months.
On present evidence oxymetholone in safe dosage has little or no role in treating the anæmia or renal failure, but trials of other anabolic steroids with less masculinising effect than testosterone should continue.
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