Studies using technique based on retrograde and anterograde transport of horseradish peroxidase were performed to address the organization of the connections of different sectors of the zone incerta (ZI) of the diencephalon with substructures of the substantia nigra, ventral tegmental field, and pedunculopontine nucleus of the midbrain in dogs. These structures were found to be interconnected. The organization of these connections included elements demonstrating that segregated conduction of functionally diverse information can be transmitted via these pathways. In addition, the convergence of projections fibers from neurons in substructures of the midbrain nucleus described in all sectors of the ZI and to these neurons from neurons in all ZI sectors is evidence that functionally diverse information can be integrated both at the level of the ZI and at the level of the midbrain structures studied here.
Studies of the pedunculopontinopallidal projections of the dog brain based on the retrograde axonal transport of horseradish peroxidase demonstrated that the compact zone (PPNc) and the lateral area of the diffuse zone (PPNd) of the pedunculopontine tegmental nucleus (PPN) of the midbrain project to the globus pallidus, entopeduncular nucleus, and ventral pallidum. The medial area of the PPNd, adjacent to the chiasm of the upper cerebellar peduncles and seen in other animals as the mesencephalic extrapyramidal area (MEA), projects only to the globus pallidus. In dogs, this area of the tegmentum is not a major source of projections to the striopallidum, such that it is inappropriate to regard it as a separate structure, comment being restricted to the topical organization of PPNd projections to the pallidum. Projection fibers to pallidal structures arise from both cholinergic and non-cholinergic PPN neurons.
A method based on retrograde axonal transport was used to undertake detailed studies of the efferent projections of individual substructures of the pedunculopontine nucleus of the midbrain tegmentum to functionally diverse segments of striatal structures in dogs. The compact and diffuse zones of this nucleus were found to project to segments of the putamen, caudate nucleus, and nucleus accumbens, which are components of the limbic and motor systems. The medial part of the diffuse zone, adjacent to the decussation of the superior cerebellar peduncles (presumptively the mesencephalic extrapyramidal field) projected only to the dorsal segments of the caudate nucleus and putamen, which are parts of the motor system.
Vital intracerebral microdialysis combined with HPLC with electrochemical detection was used to study changes in dopamine release in the nucleus accumbens during the development and realization of an emotional conditioned response in hooded rats with lesions to the hippocampal formation. These studies showed that one month after bilateral administration of ibotenic acid into the hippocampal formation, rats had weakened emotional responses to contextual stimuli. The process of development of the conditioned reflex was accompanied by higher-level and longer-lasting release of dopamine in the nucleus accumbens than in sham-operated rats. Dopamine release levels in the nucleus accumbens during realization of the conditioned reflex to contextual stimuli in rats with hippocampal lesions and sham-operated rats were identical.
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