A new, improved procedure was developed for determining dimethylphosphorothioate (DMTP) levels in the urine of workers exposed to the organophosphorus pesticide, fenitrothion. The use of ethyl acetate instead of acetonitrile/ ether to extract urine significantly increased extraction efficiency (from 57% to 96%). The use of Methelute TM (trimethylanilinium hydroxide) (TMAH) as an on-column derivatizing agent increased sensitivity 10-100-fold, was rapid, gave increased column life, and eliminated the need to work with the more hazardous diazoethane (DAE) compound used in the Shafik ethylation procedure. Total DMTP (¿ig) excreted by 3 days postexposure is reported for 16 field workers after urine analysis by both TMAH and DAE derivatization.
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