The heavy metal cadmium is very toxic to biological systems. Although its effect on the growth of microorganisms and plants has been investigated, the response of antioxidant enzymes of Aspergillus nidulans to cadmium is not well documented. We have studied the effect of cadmium (supplied as CdCl(2)) on catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). 0.005 mM CdCl(2) had a very slight stimulatory effect on the growth rate of A. nidulans, but at concentrations above 0.025 mM, growth was totally inhibited. The accumulation of Cd within the mycelium was directly correlated with the increase in the concentration of CdC(2) used in the treatments. Although a cadmium-stimulated increase in SOD activity was observed, there was no change in the relative proportions of the individual Mn-SOD isoenzymes. Higher concentrations of CdCl(2) induced a small increase in total CAT activity, but there was a major increase in one isoenzymic form, that could be separated by gel electrophoresis. GR activity increased significantly following treatment with the highest concentration (0.05 mM) of CdCl(2). The increases in SOD, CAT, and GR activities suggest that CdCl(2) induces the formation of reactive oxygen species inside the mycelia of A. nidulans.

Página 1 Efeito das dosagens de cádmio no peso seco do micélio da linhagem MSE em diferentes períodos de tempo .

pesquisadores da Embrapa, por todo o suporte na área de bioinformática estrutural; À Dra. Sabrina M. Chabregas, pesquisadora do Centro de Tecnologia Canavieira, pelo irrestrito apoio. Bem como aos doutores Eugênio C. Ulian e Maria Cristina Falco e às técnicas Rose e Daniela; Ao Prof. Dr. Giancarlo C. X. Oliveira pela orientação e pelas impagáveis conversas; Ao Prof. Dr. Márcio de Castro Filho pelas enriquecedoras discussões; Ao Prof. Dr. Cláudio Lopes, pela oportunidade e apoio fornecido nos momentos difíceis;