The effect of harvest time on the dry matter (DM) yield and quality of 10 annual temperate legumes [Trifolium subterraneum, var. brachcalycinum and var. subterraneum L. (subterranean clover), T. michelianum Savi (balansa clover), T. alexandrinum L. (berseem clover), Medicago murex Willd. (murex medic), M. truncatula Gaertn. (barrel medic), T. vesiculosum Savi (arrowleaf clover), Vicia sativa L. (common vetch), V. benghalensis L. (purple vetch), Pisum sativum L. (peas) grown in monoculture or in mixtures with oats (Avena sativa L.)] was examined in a Mediterranean environment in southern New South Wales. Three of the legumes (subterranean clover, berseem and peas) were also grown in mixtures with ryegrass (Lolium multiflorum Lam.). The forages were sown on 29 May and harvested at three times (2 October, 23 October and 6 November), corresponding to the boot, anthesis and dough stage of the cereal oat variety, Kalgan. Peas were the highest yielding of the legume monocultures at each of the three harvest times (6.9, 11.6, 12.3 t DM/ha), followed by purple vetch (6.2, 9.9, 8.7 t DM/ha). Of the small-seeded pasture legumes, berseem, murex and arrowleaf clover were the highest yielding at the second harvest (7.5–8.8 t DM/ha) when most reached their peak biomass. The inclusion of oats with the legumes increased total DM yield at the first, second and third harvests by 10–54, 7–99 and 3–102%, respectively. Oat monocultures were high yielding (up to 17.7 t DM/ha), but had low N contents (6.3–12.5 g N/kg DM). Oat–legume forage mixtures had nitrogen (N) contents of 7–15 g N/kg compared with 17–40 g N/kg in the legume monocultures. The N content and digestibility of most species and mixtures decreased as harvest time was delayed, whereas total DM peaked at the second harvest for all species except Cooba oats. The legume content of the mixtures was negatively correlated with total DM yield, DM content and water soluble carbohydrates, but positively correlated with N content, in vitro digestibility and buffering capacity. The digestibility of oats decreased more rapidly than the legumes with advancing maturity, and it would need to be harvested at the boot stage to produce a silage of sufficiently high metabolisable energy for the production feeding of cattle. A later harvest at anthesis when yield is higher would produce a silage of sufficient quality for production feeding from an oat–large seeded legume mixture with a legume content of 0.50. The oats and ryegrass had high water soluble carbohydrate and low to medium buffering capacity and would, therefore, be expected to produce well-preserved silages. The legume monocultures had low water soluble carbohydrate contents and high buffering capacity values and there would be a high risk of a poor silage fermentation in these forages without field wilting or the use of a silage additive.

Six silages were prepared from a primary growth of red clover using additives of 2 1 formic acid/t fresh crop together with formaldehyde at 0, 16, 34, 52, 77 or 117 g/kg crude protein (CP) in the crop. These silages were offered to appetite, either alone or with a urea supplement at 19-4 g/kg total dietary dry matter (D.M.), to 60 British Friesian steer calves with an initial mean live weight of 106 kg.Formaldehyde treatment restricted silage fermentation, the effect increasing with level of application. However, butyric acid and 2,3-butanediol content increased at intermediate levels of application. Protein degradation in the silages was reduced by formaldehyde treatment, as evidenced by a decline in ammonia-N and an increase in insoluble-N content with increasing level of formaldehyde application. The recovery of applied formaldehyde in the silages was low (less than 13 %) but increased with level of application.Intake, live-weight gain and feed conversion ratio followed quadratic trends, with formaldehyde having a deleterious effect at high levels of application. The decline in these production measurements was associated with declines in the apparent digestibility of D.M., organic matter (OM), N and energy with increasing level of formaldehyde application, although there was no effect of formaldehyde on cellulose digestibility. Urea supplementation tended to increase intake and live-weight gain at formaldehyde levels greater than 34 g/kg CP, and apart from the expected increase in N digestibility, did not affect the digestibility of other dietary components. As urea supplementation did not overcome the adverse effects of high levels of formaldehyde application on intake, live-weight gain and digestibility, it appears that the supply of rumen-degradable N was not the major limiting factor on these diets. N retention followed a quadratic trend with level of formaldehyde application, increasing at intermediate levels (30-50 g/kg CP) and then declining markedly at the highest level of application. A similar trend was evident when N retention was expressed as a proportion of live-weight gain, suggesting possible formaldehyde effects on carcass composition. However, carcass composition data did not confirm any formaldehyde effect. Urea supplementation did not affect N retention, and N balance data indicated poor utilization of the supplementary urea N.When considering the use of formaldehyde, relative to a formic acid control, in additives applied to red olover at ensiling, these data demonstrate little advantage in favour of formaldehyde and serious disadvantages when large quantities of formaldehyde are applied.

The influence of level of formaldehyde application at ensiling on fermentation patterns in ryegrass, red clover and maize silages was studied in three experiments using small-scale silos. Formaldehyde was applied, together with formic acid at 2 litre t-l fresh crop, over the range 0 to 166 g formaldehyde kg-1 crude protein (CP) in the crop. In ryegrass and red clover, but not in maize, low levels of formaldehyde application ( < 60 g kg-1 CP) induced clostridial-type fermentations, as evidenced by increases in the content of volatile fatty acids and ammonia N. The concentrations of ethanol and 2,3-butanediol were also increased. At higher levels of application, formaldehyde was particularly effective in restricting fermentation in ryegrass and red clover silages, there being little acid production above 120 and 80 g kg-l CP, respectively. Only low levels of formaldehyde (ca 20 g kg-1 CP) were required to achieve this effect in maize silage. In each crop the insoluble N content of the silages increased with level of formaldehyde application. The recovery of formaldehyde in both the treated herbage and silage improved with level of application, but did not exceed 50% of that applied. As physical losses of formaldehyde would be expected to be minimal under the conditions of the experiments, it appears that chemical bonding was the main reason for the lack of recovery of formaldehyde.

The effect of the application, before ensiling, of formic acid alone, and together with increasing levels of formaldehyde, on the degradability of the protein of ryegrass and red clover silages has been assessed on the basis of nitrogen solubility in mineral buffer; susceptibility of N to degradation during in-vitro incubation with either rumen microorganisms, acid pepsin or neutral protease; and N disappearance when the silages were incubated in situ in Dacron bags in the rumen of sheep receiving dried grass. The relative effects of the additives were generally consistent for both crops and with all procedures: formic acid either had no effect or reduced degradability by only a small amount, whereas a mixture of formic acid and formaldehyde was more effective than formic acid alone in protecting protein from degradation, and degradability decreased in a curvilinear manner with increasing levels of formaldehyde application. Absolute values for protein degradability based on buffer solubility and in-vitro degradation by rumen microorganisms were very similar but lower than those based on digestion with proteolytic enzymes which in turn were lower than those obtained with the rumen in situ procedure. Buffer solubility and in-vitro incubation with rumen microorganisms also showed much bigger differences between the formic acid-treated and the formaldehyde-treated silages than the other methods.

Three silages were prepared from a second cut of perennial ryegrass, using additives of 2 1 formic acid/t fresh crop together with formaldehyde at either 0, 47 or 100 g/kg crude protein (CP) in the crop. The silages were offered to appetite either alone, or with supplements of maize starch or maize starch + urea, to 18 British Friesian steer calves, half of which were rumen-cannulated. The maize starch and urea supplements were provided at 184-4 and 18-9 g/kg total dietary dry matter (D.M.) respectively.The low contents of volatile fatty acids and ammonia N in each silage indicated that they were well preserved. Formaldehyde treatment reduced protein degradation in the silages and reduced total acid content. There was a small increase in butyric acid content at the low application of formaldehyde, and the content of 2,3-butanediol increased with formaldehyde application.Formaldehyde treatment depressed intake, although the decline at the high level of application was less when calves were given the starch + urea supplement. Supplementation with starch reduced silage D.M. intake, although total D.M. intake was unaffected, and increased digestible organic matter (OM) intake. The addition of urea to the supplement further increased total D.M., digestible OM and digestible energy intakes. The apparent digestibilities of D.M., OM, N, cellulose and energy were depressed by formaldehyde treatment. Starch supplementation increased the apparent digestibility of D.M. and OM but depressed that of N and cellulose. The deleterious effect on cellulose digestibility was most evident at the high application of formaldehyde. Starch + urea supplementation further increased the digestibility of D.M. and OM, and increased N digestibility. Both supplements increased energy digestibility. N retention was improved by formaldehyde treatment, particularly when the starch -furea supplement was provided. Supplementation with starch did not improve the utilization of dietary N, but reduced N retention on the formaldehyde-treated silages.Within the rumen, ammonia-N concentration declined with increasing application of formaldehyde, and was increased by starch + urea supplementation. There was a tendency for the molar proportion of acetate to increase, and that of propionate and butyrate to decrease with increasing application of formaldehyde. Starch supplementation resulted in a small increase in the molar proportion of butyrate.The apparent rate of digestion of silage in nylon bags in the rumen was reduced by the high application of formaldehyde. On this silage diet, rate of digestion was further depressed by supplementation with starch, but improved by supplementation with starch + urea. The apparent rate of digestion was also depressed when a reference silage (the control) was incubated in the rumens of calves on the high formaldehyde silage, indicating that the adverse effect of formaldehyde was partly due to an effect on the rumen environment. However, it is also possible that formaldehyde reacted with cell wall components, inhibiting f...