SUMMARYThe sedimentation constant of simian virus type 38 (SV-38) DNA was estimated to be 3I'6S. The intrinsic viscosity of DNA was on average 86"5 dl/g and the length of the molecule determined by electron microscopy was lo.6#m. The average mol. wt., as determined by sedimentation and viscometry, was 21.5 × IO6, which agreed well with the value derived from the length of the molecule (2I. 4 × IO 6) and with the value of 21-2 × 1o 6 determined by the relative electrophoretic mobility of the DNA fragments produced by restriction endonucleases EcoRI, SalI and BgIII.The buoyant density of the DNA in caesium chloride and caesium sulphate was I'7185 and 1.4295 g/ml respectively. The melting temperature of the DNA in I × SSC was 93"5 °C. The GC content calculated fromp and Tm values was 59"3 %.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.