Aclacinomycin 10-hydroxylase is a methyltransferase homologue that catalyzes a S-adenosyl-L-methionine (AdoMet)-dependent hydroxylation of the C-10 carbon atom of 15-demethoxy-⑀-rhodomycin, a step in the biosynthesis of the polyketide antibiotic ␤-rhodomycin. SAdenosyl-L-homocysteine is an inhibitor of the enzyme, whereas the AdoMet analogue sinefungin can act as cofactor, indicating that a positive charge is required for catalysis.18 O 2 experiments show that the hydroxyl group is derived from molecular oxygen.

No abstract

No abstract