The jatropha oil was extracted from the jatropha seeds collected from different origins viz., Malaysia, Indonesia and Thailand. The physicochemical properties such as density, viscosity, percentage free fatty acid (FFA), iodine value, saponification value and peroxide value of the extracted jatropha seed oil were evaluated. The evaluation of fatty acid composition using gas chromatography (GC) revealed that, oleic (42.4-48.8%) and linoleic acid (28.8-34.6%) are the dominant fatty acids present in the jatropha seed oil. The saturated fatty acids such as palmitic and stearic acid lie in the range 13.25-14.5 and 7-7.7%, respectively. The observed major triacylglycerol (TAG) composition was OOL (22.94-25.75%) and OLL (15.52-20.77%).