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The possibility that certain types of urinary steroid conjugates might be selectively hydrolysed by boiling the urine at a neutral pH was first suggested by the work of Speirs, Wragg, Bonner & Homburger (1951), in which it was shown that chloroformextractable material, active in the mouse-eosinophil test of Speirs & Meyer (1951), could be liberated in urines by this method from patients treated with adrenocorticotrophic hormone. Further attention was drawn to this possibility by the demonstration of Teich, Rogers, Lieberman, Engel & Davies (1953) that sodium dehydroepiandrosterone (3f,-hydroxy

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