We demonstrate an inverted microscope that can image specimens in both reflection and transmission modes simultaneously with a single light source. The microscope utilizes a digital micromirror device (DMD) for patterned illumination altogether with two single-pixel photosensors for efficient light detection. The system, a scan-less device with no moving parts, works by sequential projection of a set of binary intensity patterns onto the sample that are codified onto a modified commercial DMD. Data to be displayed are geometrically transformed before written into a memory cell to cancel optical artifacts coming from the diamond-like shaped structure of the micromirror array. The 24-bit color depth of the display is fully exploited to increase the frame rate by a factor of 24, which makes the technique practicable for real samples. Our commercial DMDbased LED-illumination is cost effective and can be easily coupled as an add-on module for already existing inverted microscopes. The reflection and transmission information provided by our dual microscope complement each other and can be useful for imaging non-uniform samples and to prevent self-shadowing effects.

In computational imaging by pattern projection, a sequence of microstructured light patterns codified onto a programmable spatial light modulator is used to sample an object. The patterns are used as generalized measurement modes where the object information is expressed. In this Letter, we show that the resolution of the recovered image is only limited by the numerical aperture of the projecting optics regardless of the quality of the collection optics. We provide proof-of-principle experiments where the single-pixel detection strategy outperforms the resolution achieved using a conventional optical array detector for optical imaging. It is advantageous in the presence of real-world conditions, such as optical aberrations and optical imperfections in between the sample and the sensor. We provide experimental verification of image retrieval even when an optical diffuser prevents imaging with a megapixel array camera.

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