A device commonly used for cell electrophoresis is the cytopherometer. Its application in an immunological tumor test, however, pointed out many shortcomings and instabilities. In order to use cytopherometers in cellular immunology, a procedure was worked out to stabilize this instrument. By enlarging the electrode chambers, using a simple and efficient buffer in all compartments, coating the electrophoresis chamber, and by following a set of electrophoretic parameters (current, temperature, etc.), an intraassay variance of 1.8 % and an interassay variance of approximately 1 % were reached. I 1 Plasma from normal male and female rats was analyzed, after removal of albumin by affinity chromatography, by two-dimensional electrophoresis. The results demonstrated gender differences in two plasma proteins with apparent molecular weights of 70 000 and 84 000 and isoelectric points ranging from 6.2 to 6.4, and 5.1 to 5.5, respectively. Both proteins were found to be more concentrated in the plasma minus albumin (P-alb) fraction of females than males in both Wistar and Sprague-Dawley rats. The 70000 protein is serum albumin which was more completely removed from male plasma than female plasma. A possible mechanism responsible for this difference is discussed. The results support the existence of "maleness" and "femaleness" in blood protein patterns of rats.
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