The objective of this work was to evaluate pregnancy rates in buffaloes subjected to fixed-time AI (FTAI) during the year in Argentina. The data analysed was collected between 2016 to 2019 in a farm located in Paso Florentin City (27°20′33″ latitude and 58°08′27″ longitude) in Corrientes, Argentina. The animals were grazing native pastures (Andropogon lateralis, Paspalum almum). Ten fixed-time AI (FTAI) sessions were performed, 6 within the favourable season (BS, March–June) and 4 in the outbreeding season (OBS; September–December). Murrah and Mediterranean breed (n=141) buffaloes were used. All animals were healthy, without anatomical alterations and with normal reproductive tracts. Heifers (H), adult females with calf (AFC), and dry buffaloes (DB) were used. The average (±s.d.) weight of the animals were 459.0±37.5kg, 581.3±53.6kg, and 583.3±53.1kg, respectively. The presence of corpus luteum (CL) or a dominant follicle (≥8mm) at the beginning of the protocol was recorded. BS ovulation induction: Ovsynch (n=182); Day 0 gonadotrophin-releasing hormone (GnRH; 100μg, busereline acetate), Day 7 prostaglandin F2α (150μg, cloprostenol), Day 9 GnRH and FTAI 16h after the last GnRH injection. For the OBS (n=85): Day 0 intravaginal device (700mg of P4) + oestradiol benzoate (0.2g), Day 8 removal of the device + prostaglandin F2α + oestradiol cypionate (0.1g) + eCG (400IU) and Day 10 FTAI (52–56h) after device removal. Semen from 5 bulls of proven fertility was used, and the same technician performed all the inseminations. A specifically designed format to record the data were used and the average±s.d. of the physiological and the meteorological parameters were calculated. Pregnancy was detected 40 days after FTAI using ultrasonography. Comparison within variables were performed using ANOVA and Tukey test, with InfoStat-Statistical Software. A total of 267 inseminations were performed, and the overall pregnancy rate (PR) was 37.45% (100/267). Comparing BS with OBS, PR were 40.7% (74/182) and 30.6% (26/85) (P<0.005). Heifers had a higher PR (51.5%) than suckled buffaloes (38.8%) and dry buffaloes (20%) during the BS (P<0.05). In the OBS, there were no differences among categories on PR for [36.36 (4/11) H, 28.57 (12/42) AFC, 19.51 (8/41) DB; P>0.05]. The presence of a CL at the beginning of the FTAI protocol had a positive effect on PR during the year in all categories [31.79% (55/173) H, 40.42 (38/94) AFC, 21.51 (17/79) DB vs. 25.45% (28/110) H, 29.68 (19/64) AFC, 19.56 (9/46) DB; P<0.05]. Season had a significant effect over PR, but rain, temperature, daylight, and humidity did not differ during the year. These results show the feasibility of FTAI in water buffalo, confirm that it is possible to maintain the PR during the year, and demonstrate successful performance of protocol induction in cyclic females during the year. More research is needed to improve PR in buffaloes in Argentina, to explain the low results in suckled and dry adult buffaloes, and to analyse the effect of meteorological conditions over the breeding performance of the animals.
It is well known that buffaloes have seasonal anoestrus and that the use of melatonin reduces the effects of season in reproduction, articularly among sheep and goats. This study evaluates the use of melatonin on water buffaloes to increase pregnancy rates in a fixed-time insemination programme. The study was conducted on an Argentinian buffalo herd, located in the Corrientes Province, during an unfavourable reproductive season. Artificial insemination cycles were performed in September and December 2018. Sixty animals were selected, and melatonin was administered to 29 females at a dose of 18 mg/50 kg. Ten days after the melatonin implant, ovulation synchronization was started. Animals were inseminated with frozen semen of a single bull. Pregnancy was evaluated by ultrasound 35 days after insemination. The pregnancy rate of the two repetitions was 34.62% for September and 6.4% for December (P=0.007). No significant differences were found in the parameters associated with follicular development and formation of the corpus luteum. Analysis of the effects of melatonin on the two replicates showed that there was no statistically significant effect of ovarian response regarding the analysed follicular development parameters. There was also no effect on pregnancy rates (17.85% vs. 20.68%; P=0.15), or on follicular development for treatment and control. In conclusion, this study was not able to demonstrate an effect of melatonin on the ovarian response of buffaloes as reported by other authors. Other factors, such as environmental conditions of subtropical areas and species specificities, may have a more significant effect on buffalo endocrinology. These factors should be evaluated to improve the results of fixed-timed artificial insemination programmes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.