Crossbred steers (n = 7; 400 kg BW), fitted with T-type cannulas in the duodenum and ileum, were used to examine the effects of processing method, dry-rolled (DR) vs. steam-flaked (SF) sorghum grain, and degree of processing (flake density; FD) of SF corn (SFC) and SF sorghum (SFS) grain on site and extent of DM, starch, and N digestibilities and to measure extent of microbial N flow to the duodenum. In Exp. 1, diets contained 77% DRS or 77% SFS with FD of 437, 360, and 283 g/L (SF34, SF28, and SF22). In Exp. 2, diets contained 77% SFC with FD of SF34 or SF22. For sorghum and corn diets, respective average daily intakes were as follows: DM, 6.7 and 8.1 kg; starch, 3.8 and 4.7 kg; N, 136 and 149 g. Steers fed SFS vs. DRS increased (P = .01) starch digestibilities (percentage of intake) in the rumen (82 vs. 67%) and total tract (98.9 vs. 96.5%) and decreased digestibilities in the small intestine (16 vs. 28%; P = .01) and large intestine (.5 vs 1.2%; P = .05). As a percentage of starch entering the segment, digestibility was increased (P = .01) within the small intestine (91 vs. 85%) but was not altered within the large intestine by steers fed SFS vs. DRS. Decreasing FD of SFS and of SFC, respectively, linearly increased starch digestibilities (percentage of intake) in the rumen (P = .03, .02) and total tract (P = .03, .09) and linearly diminished starch digestibilities in the small intestine (P = .04, .09). Starch digestibilities (percentage of entry) within the small or large intestine were not changed by FD. The percentage of dietary corn or sorghum starch digested in the large intestine was very small, less than 2% of intake. Microbial N flow to the duodenum was not altered by SFS compared to DRS, or by decreasing FD of SFS and SFC. Reducing FD of SFS, but not of SFC, tended to decrease (P = .07) microbial efficiency linearly and tended to increase (P = .06) total tract N digestibilities linearly. Steam flaking compared to dry rolling of sorghum grain and decreasing FD of SFC and SFS grain consistently increased starch digestibility in the rumen and total tract of growing steers. The greatest total digestibility of dietary starch occurred when the proportion digested in the rumen was maximized and the fraction digested in the small intestine was minimized. These changes in sites of digestion account, in part, for the improved N conservation and greater hepatic output of glucose by steers fed lower FD of SFS reported in our companion papers.

The objective of this study was to determine effects of processing method, dry-rolled (DR) vs steam-flaked (SF), and degree of processing (flake density, FD) of SF sorghum grain on splanchnic (gut and liver) N metabolism by growing steers. Diets contained 77% sorghum grain either DR or SF at densities of 437, 360, and 283 g/L (SF34, SF28, and SF22, respectively). Eight crossbred steers (340 kg initial BW), implanted with indwelling catheters into portal, hepatic, and mesenteric veins and the mesenteric artery, were used in a randomized complete block design. Blood flows and net output or uptake of ammonia N, urea N (UN), and alpha-amino N (AAN) were measured across portal-drained viscera, hepatic, and splanchnic tissues. Plasma arterial, portal, and hepatic concentrations of individual amino acids were also measured. Decreasing FD linearly increased (P = .04) net absorption of AAN (51, 73, and 78 g/d for SF34, SF28, and SF22, respectively) and transfer (cycling) of blood UN to the gut (49, 48 and 64 g/d; P = .02). Net UN cycling averaged 38% of N intake across all diets. Hepatic uptake of AAN or UN synthesis, and splanchnic output of AAN and UN, were not altered by FD. Lowering FD linearly increased (P < or = .02) portal-arterial concentration differences for blood AAN and UN and plasma arterial concentrations for alanine. Steers fed SF compared to DR tended to have greater (P = .11) blood UN cycling (percentage of hepatic synthesis; 64 vs 50%) and decreased (P = .03) net splanchnic UN output (30 vs 50 g/d), but other net fluxes of N were not altered across splanchnic tissues. Steam-flaking compared to dry-rolling tended to decrease (P = .12) portal, but not hepatic, blood flow and increased (P < .01) hepatic-arterial concentration differences for blood UN. Except for a decrease (P = .01) in hepatic-arterial concentration differences of glutamine, plasma amino acid concentrations were not altered by feeding SF vs DR sorghum. Processing method (steam-flaking vs dry-rolling) or increasing the degree of processing (by decreasing FD) of SF sorghum grain resulted in greater transfer of blood UN to the gut. Reducing FD also linearly increased the absorption of AAN by growing steers, which explains (in part) published responses of superior performance by steers fed SF grains.

Our objectives were to measure net fluxes of free AA (FAA) and peptide-bound AA (PBAA) across portal-drained viscera, liver, splanchnic tissues, and mammary tissues, and milk AA output of lactating Holstein cows (n = 8, 86 +/- 8 d in milk). Cows were fed an alfalfa-based total mixed ration containing 40% steam-flaked (SFS) or dry-rolled (DRS) sorghum grain. The total mixed rations were offered at 12-h intervals in a crossover design. Blood samples were obtained from indwelling catheters in portal, hepatic, and mammary veins and from mesenteric or costoabdominal arteries every 2 h from each cow and diet. Intake of dry matter was 17.9 and 18.6 kg/d of the SFS and DRS diets, respectively, but dropped to 16.3 kg/d for cows fed the SFS diet in the last 3 experimental days, sampling day included. Milk and milk crude protein yields (kg/12-h sampling) were 13.85 vs. 13.25 and 0.425 vs. 0.396 for cows fed SFS or DRS, respectively, and were not affected by the considerable drop in dry matter intake of cows fed the SFS diet during the last 3 experimental days. The portal-drained visceral flux of total essential FAA was 417 and 442 g/12 h (SEM 63) in cows fed SFS and DRS, respectively. However, the portal-drained visceral flux of 7 essential PBAA out of the 9 determined was numerically greater in cows fed the SFS diet, and total essential PBAA in that treatment was 77.4 +/- 22.2 compared with 35.4 +/- 50.2 g/12 h for cows fed the DRS diet. This phenomenon was again observed in a greater total splanchnic flux (FAA + PBAA) of 462 and 371 g/12 h in SFS- and DRS-fed cows, respectively. Mammary uptake of essential AA from both pools (free and peptide bound), and recovery of essential AA in milk, was again numerically higher in SFS-fed cows. In addition to FAA, quantifying the contribution of PBAA may improve our understanding of tissue use of AA substrates, and this may ultimately lead to improved diet formulations with respect to intestinal absorption and mammary uptake of AA.

Objectives were to measure net fluxes of free (FAA) and peptide bound amino acids (AA) (PBAA) across portal-drained viscera (PDV), liver, splanchnic, and mammary tissues, and of milk AA output of lactating Holstein cows (n = 6, 109 +/- 9 d in milk) as influenced by flaking density of corn grain. Cows were fed alfalfa-based total mixed ration (TMR) containing 40% steam-flaked (SFC) or steam-rolled corn (SRC) grain. The TMR were offered at 12-h intervals in a crossover design. Six sets of blood samples were obtained from indwelling catheters in portal, hepatic, and mammary veins and mesenteric or costoabdominal arteries every 2 h from each cow and diet. Intake of dry matter (18.4 +/- 0.4 kg/d), N, and net energy for lactation were not altered by corn processing. Milk and milk crude protein yields (kg/12-h sampling) were 14.2 vs. 13.5 and 0.43 vs. 0.39 for cows fed SFC or SRC, respectively. The PDV flux of total essential FAA was greater (571.2 vs. 366.4 g/12 h, SEM 51.4) in cows fed SFC. The PDV flux of total essential PBAA was 69.3 +/- 10.8 and 51.5 +/- 13.2 g/12 h for cows fed SFC and SRC, respectively, and differed from zero, but fluxes of individual PBAA rarely differed between treatments. Liver flux of essential FAA was greater in cows fed SRC, but only the PBAA flux in cows fed SRC differed from zero. Splanchnic flux of FAA and PBAA followed the pattern of PDV flux, but variation was greater. Mammary uptake (g/12 h) of total essential FAA was greater in cows fed SFC than SRC (224.6 vs. 198.3, SEM 7.03). Mammary uptake of essential PBAA was 25.0 vs. 15.1, SEM 5.2, g/12 h for cows fed SFC or SRC, respectively, and differed from zero in half of the PBAA. Milk output of EAA was 187.8 vs 175.4, SEM 4.4 g/12 h in cows fed SFC and SRC, respectively, and output of most essential AA consistently tended to be greater in cows fed SFC. It is apparent that PBAA comprise a portion of total AA flux across PDV and are affected by grain processing. Further, this pool supplies an important component of AA taken up by the mammary gland. Quantifying the contribution of PBAA may improve diet formulation with respect to intestinal absorption and mammary uptake of AA.

Objectives were to determine net release or uptake of alpha-amino N, ammonia N, and urea N across portal-drained viscera, liver, splanchnic, and mammary tissues of lactating Holstein cows (n = 8, 86 +/- 8 d in milk) fed alfalfa hay-based total mixed rations containing 40% dry-rolled or steam-flaked sorghum grain. The total mixed rations were offered at 12-h intervals in a crossover design. Blood samples were obtained from indwelling catheters in the portal, hepatic, and mammary veins and mesenteric or costoabdominal arteries, every 2 h for each cow and diet. Steam-flaking increased in vitro rate of starch hydrolysis compared with dry-rolled sorghum (66 vs. 25%). Diet did not alter dry matter intake (18.2 +/- 0.3 kg). Daily milk yield (27.6 +/- 0.8 kg), efficiency of production, and most milk components did not differ between diets, but fat yield was reduced (0.86 vs. 0.91 kg/d) by steam-flaked sorghum, and lactose concentration was increased (4.99 vs. 4.82%). Blood flows in portal and hepatic veins did not differ between diets. Steam-flaking tended to increase urea N cycling to the gut (162 vs. 95 g/d) compared with dry-rolling of sorghum, whereas net absorption of ammonia N and alpha-amino N across portal-drained viscera were decreased. Net mammary uptake of a-amino N increased more than 20% (83 vs. 67 g/d), resulting in a higher mammary extraction ratio (15 vs. 11%) for steam-flaked versus dry-rolled sorghum. Flaking of sorghum improved the efficiency of postabsorptive N metabolism by increasing urea N cycled to the gut and alpha-amino N uptake by the mammary gland.