Randomly amplified polymorphic DNA (RAPD) markers were used to estimate intra- and interspecific variations in the genus Lens (lentil). Twenty cultivars of L. culinaris ssp. culinaris, including 11 microsperma (small-seeded) and nine macrosperma (large-seeded) types, and 16 wild relatives (four accessions each of L. culinaris ssp. orientalis, L. odemensis, L. nigricans and L. ervoides), were evaluated for genetic variability using a set of 40 random 10-mer primers. Fifty reproducibly scorable DNA bands were observed from ten of the primers, 90% of which were polymorphic. Genetic distances between each of the accessions were calculated from simple matching coefficients. A dendrogram showing genetic relationships between them was constructed by an unweighted pair-group method with arithmetical averages (UPGMA). This study revealed that (1) expect for L. ervoides, the level of intraspecific variation in cultivated lentil is lower than that in wild species, (2) L. culinaris ssp. orientalis is the most likely candidate for a progenitor of the cultivated species, and (3) microsperma and macrosperma cultivars were indistinguishable by the RAPD markers identified here.
Path coefficient analyses were applied to detect the direct and indirect effects upon grain yield/plant in three groups as a: each of seed index, biological yield/plant and weight of spikes/plant; b: each of thrashing index, biological yield/plant and weight of spikes/plant; and c: all studied grain yield attributes. population of 5 and F 4The three groups of path analyses were applied through F early and late pedigree line selection . The direct effects of biological yield and weight of spikes spikes/plant were decreased from the starting generations to final selections in the three groups, except final early selections possessed increased values in group two (g2) and group three (g3) for weight of spikes/plant. Otherwise, the direct effect of harvest index on grain yield was increased from started base to final selections. These results exhibited that the direct effects of those three traits on grain yield were responded differently according to the rearrangement of their genetic make-up across the different generations of selections and the type of path analysis.
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