The purpose of the study was to substantiate the effectiveness of local combined use of miliacil and EHF-therapy in the complex treatment of purulent wounds of the lower extremities in patients with diabetes mellitus and its effect on morphological and functional changes in tissue defects. Our studies included 96 patients with purulent wounds of the lower extremities, localized on the thigh or lower leg against the background of diabetes mellitus. In 48 patients of the main group, miliacil and EHF-therapy were used in the local treatment of wounds, 48 patients in the control group used only miliacil. Of the 96 patients, 56 patients received complex treatment of purulent wounds, 40 received conservative treatment and plastic surgery. In all patients receiving complex conservative treatment, morphological studies of wound tissues were performed at admission, on the 7th day of conservative treatment, with the consent of the patients. When miliacil and EHF-therapy were used, the wounds healed by 13 days in most patients. Most patients in the control group showed complete wound healing by the 18th day of treatment. The positive effect of miliacil and EHF-therapy on the course of purulent wounds in patients with diabetes mellitus was also confirmed by histological research methods. An increase in leukocyte and macrophage reactions, a more active formation of poorly differentiated granulation connective tissue due to the intensification of vasculogenesis. The new method of therapeutic correction contributed to better and adequate epithelialization of the wound defect, traced after 7 days of clinical observation. Thus, with the complex use of miliacil and EHF-therapy, its high efficiency was established in the treatment of purulent wounds against the background of diabetes mellitus.
The aim of the study was to comprehensively assess histological parameters of the hypothalamic neurosecretory and immune systems in pregnant rats exposed to passive smoking and their offspring.Material and methods. We studied morphological and immunological parameters of pregnant Wistar rats exposed to passive smoking and those of the control group, as well as their offspring. The obtained material was processed using histological, immunohistochemical, morphometric and immunological methods.Results. The results obtained demonstrated that in rats exposed to passive smoking, the sizes of neurosecretory cells (NSCs) of the supraoptic (SO) and paraventricular (PV) nuclei of the hypothalamus increased, the number of p53 positive NSCs increased, and bcl-2 protein expression decreased. Tobacco smoking caused formation of a proaptotic dominant in the neurosecretory cells of the supraoptic and paraventricular nuclei of the hypothalamus. Passive smoking led to a decreased body weight, a decreased number of thymocytes and myelokaryocytes in pregnant rats. In young rats born from the animals exposed to passive smoking, there was a slowdown in the processes of postnatal differentiation of the adrenal cortex (fascicular zone) with preservation of the extended zone of the fetal cortex. Notably, histo- and morphogenesis both in the organs of the primary (thymus) and secondary (spleen, lymph nodes) links of immunogenesis were delayed. In such young rats, a decreased body weight, thymus, number of thymocytes and splenocytes were recorded.Conclusion. Total results of the study evidence that passive smoking causes immunosuppressive changes in pregnant rats and their offspring combined with delayed postnatal histogenesis and proapoptotic manifestations in the nonapeptide-dergic hypothalamicpituitary adrenocortical system, which can be regarded as an unfavorable factor in the implementation of the neuroendocrine regulative mechanisms of adaptogenesis processes.
Objective - development of Klebsiella - Candida arthritis model in Wistar rats. Material and methods. The study was carried out on 20 male Wistar rats, of which 15 animals were injected with a bacterial-fungal suspension by introducing into the cavity of the hock (tarsus) joint Klebsiella-Candida suspension (1: 1) with a microorganism concentration of 106 CFU / ml , using museum strains Klebsiella pneumoniae ICIS - 278 and Candida albicans ATCC 24433 under the control of an X-ray apparatus FireCR +. Five intact animals served as control. On the 15th day of the experiment, after euthanasia, a bacteriological and histological examination of the affected joints was carried out while maintaining their topography. Results. In rats infected with Klebiella-candidiasis suspension, pain syndrome and functional changes typical for arthritis were noted on days 2-3. Bacteriological examination of the affected joints in 75% of rats isolated cultures of Klebsiella pneumoniae, identical, according to PCR, to the original strain, which is part of the bacterial-fungal suspension. At the same time, fungi of the genus Candida, also used in the experiment for infecting animals, were not detected from the affected joints of rats. The reproduced model of Klebsiella-Candida arthritis is characterized by pathomorphological signs of panarthritis, accompanied by purulent inflammation of all joint structures (synovial membrane, cartilage and bone tissue) with the involvement of soft periarticular tissues. Conclusions. The presented model of Klebsiella-fungal arthritis can be used to test new means of antimicrobial (antibacterial and antifungal) therapy, which are an integral component of the treatment of arthritis associated with infection.
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