The present work was designed to study the effect of DEAE-dextran and Magnesium Chloride (MgCl) on fowl pox virus vaccine (FPV) in order to obtain a maximum titer allowing the massive production of the vaccine. Fowl pox virus; used for vaccine production; was propagated on embroynated chicken eggs (ECE) and chicken embryo fibroblast (CEF), the virus was inoculated in ECE and CEF without DEAEdextran nor MgCl, and with DEAE-Dextran and MgCl separately. The time of harvestion was early as one day post inoculation in CEF. The virus titer were higher in case of treated inoculums with DEAEdextran and MgCl reaching (6.2 and 7.5 log 10 TCID 50 /ml respectively) on CEF and (6.2 and 6.7 Log 10 EID 50 respectively) on ECE. Fowl pox vaccines (FPV) were prepared before and after DEAE-Dextran and MgCl treatment. The application of quality control assays revealed the safety, sterility and potency of the prepared vaccines. Immune response of the prepared FPV vaccines were evaluated in chickens using virus neutrization test (VNT). It was found that, the use of DEAE-Dextran and MgCl with the recommended concentrations could result in increasing the virus titer with reduction in the time of harvestation and accordingly increase the vaccine production and decrease its cost.
Convincing seroimmunological studies were conducted to explore the influence of sheep pox vaccine on the immune response to pneumo-4 vaccine in vaccinated sheep, when it is administrated before, after and simultaneously. The experiment was conducted on eighteen susceptible sheep divided into 6 equal groups. Groups 1 and 5 were vaccinated with pneumo-4 vaccine and sheep pox vaccine respectively regarded as control vaccinated groups. Sheep pox vaccine was administered simultaneously with pneumo-4 in Group (2), before and after pneumo-4 vaccine in groups (3 and 4) respectively. The sixth group being left as a non-vaccinated control. The developed antibodies against different antigens incorporated in the used vaccines were monitored by using both serum neutralization test (SNT) and ELISA. The results showed that when sheep pox was administered one-week before vaccination with pneumo-4, the highest antibody titres were developed followed by these induced by simultaneous vaccination in group (2). So, the result explores the immunopotentiating effect of sheep pox vaccine on the immune response of sheep to pneumo-4 vaccine specially when it is administered one week before the other vaccine.
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