自适应光学在双光子显微成像技术中的应用

Chen, 章辰 Zhang; Yufeng, 高玉峰 Gao; Shiwei, 叶世蔚 Ye; Hui, 李慧 Li; Wei, 郑炜 Zheng

doi:10.3788/cjl221250

中国激光

2023

DOI: 10.3788/cjl221250

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自适应光学在双光子显微成像技术中的应用

章辰 Zhang Chen¹,

高玉峰 Gao Yufeng²,

叶世蔚 Ye Shiwei³

et al.

Abstract: Significance Twophoton microscopy (TPM) has been widely used in biological imaging owing to its submicron lateral resolution, intrinsic optical sectioning, and deep penetration abilities. TPM enables the observation of cellular and subcellular dynamics in deep live tissues within highly complex and heterogeneous environments such as the mammalian brain, thereby providing critical in situ and

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Cited by 2 publications

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从随机散斑到“浑浊透镜”——基于波前整形的散射光聚焦技术（特邀）

Liu Kaige,

Zhang Hengkang,

Fu Xing

et al. 2024

光学学报

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Significance Scattering medium is a substance commonly found in nature such as turbid atmosphere, smoke, and biological tissues. Coherent light beams propagating through scattering media will be disrupted due to random scattering effects. The wavefront will be destroyed, and the transmission direction will deviate from the original input direction and becomes chaotic. Random scattered light interference will form a particlelike intensity pattern, known as an optical speckle . In multimode fibers, due to mode dispersion and intermodal interference, a similar scattering distribution will be formed. Thus, multimode fibers are also regarded as a special class of scattering medium .Due to the scattering phenomenon, it is difficult to maintain the original spatial distribution of the light beam , and the energy is exponentially attenuated with the increasing penetration depth, which greatly limits the applications of advanced technologies such as optical tweezers, optical communications, and biomedicine in a strong scattering environment. However, in 1990, Freund proposed that light scattering in static scattering media is a deterministic linear process, a property that reveals the possibility of reutilizing the energy of the scattered light field. Due to the existence of a deterministic response relationship between incident and scattered lights, suitable input conditions can lead to the formation of the desired distribution of the output light field after passing through the scattering medium . In 2007, Vellekoop and

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从随机散斑到“浑浊透镜”——基于波前整形的散射光聚焦技术（特邀）

Liu Kaige,

Zhang Hengkang,

Fu Xing

et al. 2024

光学学报

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双光子亚衍射多焦点结构光照明显微研究

Quan Xiaojuan,

Zhang Chenshuang,

Lin Danying

et al. 2023

中国激光

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scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

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自适应光学在双光子显微成像技术中的应用

Cited by 2 publications

References 15 publications

从随机散斑到“浑浊透镜”——基于波前整形的散射光聚焦技术（特邀）

从随机散斑到“浑浊透镜”——基于波前整形的散射光聚焦技术（特邀）

双光子亚衍射多焦点结构光照明显微研究

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