神经肽Y通过Y1受体激活PI3K通路促进RAW264.7细胞中TGF-β1 的产生 周江睿, 徐拯, 蒋春雷

Abstract

Objective To examine the effect of neuropeptide Y (NPY) on TGF-β1 production in RAW264.7 macrophages. Methods Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-β1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85. Results NPY treatment could promote TGF-β1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-β1 production induced by NPY could be abolished by wortmannin pretreatment. Conclusion NPY may elicit TGF-β1 production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.

Localized retinal neuropeptide regulation of macrophage and microglial cell functionality

“…It also mediates its own production, and augments expression of its receptors on macrophages establishing a self-sustaining anti-inflammatory autocrine loop (Taherzadeh et al, 1999). In contrast NPY is more pleiotropic in regulating macrophage functionality related to the type of NPY receptor expressed (De la Fuente et al, 2001; Dimitrijević et al, 2008; Dimitrijevic et al, 2005; Zhou et al, 2008). In our results we show that NPY promotes NOS2 expression.…”

Neuropeptide Y Has a Protective Role during Murine Retrovirus-Induced Neurological Disease

“…One mechanism by which NPY may affect virus levels is by limiting the recruitment and/or infiltration of virus-infected macrophages into the brain. NPY has multiple reported effects on macrophages, including inhibiting chemotaxis and modulating phagocytosis, oxidative burst, and tumor growth factor beta production (11,13,38). It is possible that NPY expression in the periphery affects macrophage activation and limits the migration of infected cells to the brain.…”

The neuropeptides α-MSH and NPY modulate phagocytosis and phagolysosome activation in RAW 264.7 cells

“…These neuropeptides with other immunomodulating factors are constitutively produced within the ocular microenvironment and are part of the mechanisms of ocular immune privilege (Taylor, 2009). Both α-MSH and NPY are considered immunomodulating neuropeptides that can influence macrophages functionality and responses to signals of innate immunity (Dimitrijević et al, 2005; Lam et al, 2006; Li and Taylor, 2008; Taylor, 2005; Zhou et al, 2008). Together they suppress proinflammatory signals and promote an alternative activation of macrophages.…”