Abstract:The development of the expression, renaturation and purification of proteins sourcing E. coli by protein folding liquid chromatography (PFLC) in past two years was briefly reviewed. PFLC has been widely employed for the separation and purification of inclusion body proteins. Besides the separation of the target protein from impure proteins, it has a character that the inclusion body proteins can be simultaneous renaturation. The preparative method of the most novel proteins employed in the modern biotechnology… Show more
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