一般演題「一般口演12　その他1」

Principles for rational Cas13d guide design

“…* * * Type VI CRISPR enzymes have recently been identified as programmable RNA-guided, RNA-targeting Cas proteins with nuclease activity that allow for target gene knock-down without altering the genome. In addition to target RNA knock-down 1-9 , Cas13 proteins have been used to enable viral RNA detection systems 7,9-11 , site-directed RNA editing 12 , demethylation of m 6 Amodified transcripts 13 , RNA live-imaging 14,15 , and modulation of splice site choice as well as cleavage and polyadenylation site usage 5,16,17 .Cas13 proteins are guided to their target RNAs by a single CRISPR RNA (crRNA) composed of a direct repeat (DR) stem loop and a spacer sequence (guide RNA) that mediates target recognition by RNA-RNA hybridization. Although Cas13 enzymes exert some non-specific collateral nuclease activity upon activation 4-6,10,18 , they have greatly reduced off-target activity in cultured cells compared to RNA interference 2,5,12 .…”

“…* * * Type VI CRISPR enzymes have recently been identified as programmable RNA-guided, RNA-targeting Cas proteins with nuclease activity that allow for target gene knock-down without altering the genome. In addition to target RNA knock-down 1-9 , Cas13 proteins have been used to enable viral RNA detection systems 7,9-11 , site-directed RNA editing 12 , demethylation of m 6 Amodified transcripts 13 , RNA live-imaging 14,15 , and modulation of splice site choice as well as cleavage and polyadenylation site usage 5,16,17 .…”